Mechanical properties of fibroblasts depend on level of cancer transformation

• Published in: Biochimica et biophysica acta Vol. 1843; no. 5; pp. 1013 - 1019
• Main Authors: Efremov, Yu.M., Lomakina, M.E., Bagrov, D.V., Makhnovskiy, P.I., Alexandrova, A.Y., Kirpichnikov, M.P., Shaitan, K.V.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2014
• Subjects: AFM; Cell Transformation, Neoplastic; Fibroblast; Fibroblasts - cytology; Focal Adhesions; Force spectroscopy; Humans; Microscopy, Atomic Force; Microscopy, Fluorescence; Transformed cell; Young's modulus; AFM; Young's modulus; Force spectroscopy; Fibroblast; Transformed cell
• ISSN: 0167-4889; 0006-3002; 1879-2596
• DOI: 10.1016/j.bbamcr.2014.01.032

Recently, it was revealed that tumor cells are significantly softer than normal cells. Although this phenomenon is well known, it is connected with many questions which are still unanswered. Among these questions are the molecular mechanisms which cause the change in stiffness and the correlation between cell mechanical properties and their metastatic potential. We studied mechanical properties of cells with different levels of cancer transformation. Transformed cells in three systems with different transformation types (monooncogenic N-RAS, viral and cells of tumor origin) were characterized according to their morphology, actin cytoskeleton and focal adhesion organization. Transformation led to reduction of cell spreading and thus decreasing the cell area, disorganization of actin cytoskeleton, lack of actin stress fibers and decline in the number and size of focal adhesions. These alterations manifested in a varying degree depending on type of transformation. Force spectroscopy by atomic force microscopy with spherical probes was carried out to measure the Young's modulus of cells. In all cases the Young's moduli were fitted well by log-normal distribution. All the transformed cell lines were found to be 40–80% softer than the corresponding normal ones. For the cell system with a low level of transformation the difference in stiffness was less pronounced than for the two other systems. This suggests that cell mechanical properties change upon transformation, and acquisition of invasive capabilities is accompanied by significant softening. •Young's modulus of transformed and normal fibroblasts was measured by AFM.•The transformed cells were significantly softer than the control ones.•For cells with higher level of transformation the softening was more pronounced.•Log-normal distribution of the Young's modulus values was shown for all cell lines.