Chitin-Triggered MAPK Activation and ROS Generation in Rice Suspension-Cultured Cells

Suspension-cultured cells respond sensitively to a number of stimuli including pathogen-derived molecules. Therefore, they are used in a simple, single-cell model system in order to gain insights into immune signaling pathways in rice. Here we describe protocols for studying chitin-induced MAPK acti...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1578; p. 309
Main Authors Yamaguchi, Koji, Kawasaki, Tsutomu
Format Journal Article
LanguageEnglish
Published United States 01.01.2017
Subjects
Cells, Cultured
Chitin - pharmacology
Enzyme Activation
Gene Expression Regulation, Plant
Mitogen-Activated Protein Kinases - metabolism
Oryza - cytology
Oryza - metabolism
Plant Immunity
Plant Proteins - metabolism
Reactive Oxygen Species - metabolism
Single-Cell Analysis - methods
Chitin
MAPK
Immunity
ROS
Rice
Online AccessGet more information

Cover

More Information
Summary:Suspension-cultured cells respond sensitively to a number of stimuli including pathogen-derived molecules. Therefore, they are used in a simple, single-cell model system in order to gain insights into immune signaling pathways in rice. Here we describe protocols for studying chitin-induced MAPK activation and ROS generation in rice suspension-cultured cells.
ISSN:1940-6029
DOI:10.1007/978-1-4939-6859-6_26