Proteomics approaches to characterize the immune responses in cancer

• Published in: Biochimica et biophysica acta. Molecular cell research Vol. 1869; no. 8; p. 119266
• Main Authors: Urbiola-Salvador, Víctor, Miroszewska, Dominika, Jabłońska, Agnieszka, Qureshi, Talha, Chen, Zhi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2022
• Subjects: Cancer; Humans; Immunity; Immunotherapy; Neoplasms - metabolism; Proteomics; Single-cell; T cells; Tumor Microenvironment - genetics; ILC; TAM; ALN; IHC; nanoPOTS; MHC; RPPA; FASLG; SCoPE-MS; Immunity; LC-MS/MS; NF-κB; IFN-γ; PLAYR; Th; FFPE; MC; mPOP; Immunotherapy; IC; IMC; AML; BAFF; ARG2; REAP-seq; IL; CAC; MALDI-TOF; CITE-seq; MS; scATAC-seq; MELC; PBMC; EV; GAM; SAA; TGF; CRC; LCM; SCBC; NGS; CyTOF; FC; NK; PD-1; SIRT; IgE; MIBI; NAPPA; PTM; SISPROT; mIF; T cells; Single-cell; Tim-3; GBM; LAG-3; PD-L1; MIST; CTLA-4; ESI; SN; ABCD; FoxP3; CODEX; CD; MSI; Treg; 2-DE; FSFC; CCL; PEA; TME; mAb; APC; TIL; Proteomics; LAP; SUGAR-seq; Cancer
• ISSN: 0167-4889; 1879-2596
• DOI: 10.1016/j.bbamcr.2022.119266

Despite the dynamic development of cancer research, annually millions of people die of cancer. The human immune system is the major ‘guard’ against tumor development. Unfortunately, cancer cells have the ability to evade the immune system and continue to grow. The proper understanding of the intricate immune response in tumorigenesis remains the holy grail of cancer immunology and designing effective immunotherapy. To decode the immune responses in cancer, in recent years, proteomics studies have received considerable attention. Proteomics studies focus on the detection and quantification of proteins, which are the effectors of biological functions, and as such, are proven to reflect the cell state more accurately, in comparison to genomic or transcriptomic studies. In this review, we discuss the proteomics studies applied to characterize the immune responses in cancer and tumor immune microenvironment heterogeneity. Further, we describe emerging single-cell proteomics approaches that have the potential to be applied in cancer immunity studies. •Proteomics approaches enable to uncover novel insights in cancer immunology.•Mass spectrometry unveils immune mechanisms and novel immunotherapeutic targets.•Antibody-based strategies determine predictive biomarker panels for immunotherapy.•Single-cell proteomics is a key to understanding the tumor microenvironment.•Single-cell multi-omics will revolutionize the studies on cancer immunology.