Three-dimensional imaging reveals that positions of cyst nematode feeding sites relative to xylem vessels differ between susceptible and resistant wheat

• Published in: Plant cell reports Vol. 40; no. 2; pp. 393 - 403
• Main Authors: Levin, Kara A., Tucker, Matthew R., Strock, Christopher F., Lynch, Jonathan P., Mather, Diane E.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.02.2021; Springer Nature B.V
• Subjects: Ablation; Animals; Biomedical and Life Sciences; Biotechnology; Cell Biology; Cell Wall - parasitology; Cell Wall - ultrastructure; Cell walls; Chromosomes; Confocal microscopy; cyst nematodes; Disease Resistance - genetics; Disease Susceptibility; Feeding; Genetic Loci; Heterodera avenae; Host plants; Imaging, Three-Dimensional; Laser ablation; Life Sciences; Lignin; Loci; Nematodes; Original Article; Plant Biochemistry; Plant Diseases - parasitology; Plant Diseases - prevention & control; Plant Proteins - genetics; Plant Proteins - metabolism; Plant resistance; Plant roots; Plant Roots - genetics; Plant Roots - parasitology; Plant Roots - ultrastructure; Plant Sciences; Plant tissues; Roots; Stain; Thickening; Tomography; Triticum - genetics; Triticum - parasitology; Triticum - ultrastructure; Triticum aestivum; Tylenchida - physiology; Vessels; Wheat; Xylem; Xylem - genetics; Xylem - parasitology; Xylem - ultrastructure; Confocal microscopy; Laser ablation tomography; Cereal cyst nematode; Heterodera avenae; Triticum aestivum
• ISSN: 0721-7714; 1432-203X; 1432-203X
• DOI: 10.1007/s00299-020-02641-w

Key message Resistance conferred by the Cre8 locus of wheat prevents cereal cyst nematode feeding sites from reaching and invading root metaxylem vessels. Cyst nematodes develop syncytial feeding sites within plant roots. The success of these sites is affected by host plant resistance. In wheat ( Triticum aestivum  L.), ‘ Cre ’ loci affect resistance against the cereal cyst nematode (CCN)  Heterodera avenae . To investigate how one of these loci ( Cre8 , on chromosome 6B) confers resistance, CCN-infected root tissue from susceptible (− Cre8 ) and resistant (+ Cre8 ) wheat plants was examined using confocal microscopy and laser ablation tomography. Confocal analysis of transverse sections showed that feeding sites in the roots of − Cre8 plants were always adjacent to metaxylem vessels, contained many intricate ‘web-like’ cell walls, and sometimes ‘invaded’ metaxylem vessels. In contrast, feeding sites in the roots of + Cre8  plants were usually not directly adjacent to metaxylem vessels, had few inner cell walls and did not ‘invade’ metaxylem vessels. Models based on data from laser ablation tomography confirmed these observations. Confocal analysis of longitudinal sections revealed that CCN-induced xylem modification that had previously been reported for susceptible (− Cre8 ) wheat plants is less extreme in resistant (+ Cre8 ) plants. Application of a lignin-specific stain revealed that secondary thickening around xylem vessels in CCN-infected roots was greater in + Cre8  plants than in − Cre8  plants. Collectively, these results indicate that  Cre8  resistance in wheat acts by preventing cyst nematode feeding sites from reaching and invading root metaxylem vessels.