Molecular cloning and developmental expression of a zebrafish axonal glycoprotein similar to TAG-1

• Published in: Mechanisms of development Vol. 80; no. 2; pp. 197 - 201
• Main Authors: Warren, James T., Chandrasekhar, Anand, Kanki, John P., Rangarajan, Radha, Furley, Andrew J., Kuwada, John Y.
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.02.1999
• Subjects: Animals; Axon pathfinding; Brain; Branchiomotor neuron; Cell adhesion molecule; Cell Adhesion Molecules, Neuronal; Central Nervous System - embryology; Central Nervous System - metabolism; Cloning, Molecular; Contactin 2; Embryo, Nonmammalian - metabolism; Embryo, Nonmammalian - ultrastructure; Eye; Gene expression; Gene Expression Regulation, Developmental; Glycoprotein; Growth cone; In Situ Hybridization; Lateral line; Membrane Glycoproteins - biosynthesis; Membrane Glycoproteins - genetics; Membrane Glycoproteins - physiology; Morphogenesis - genetics; Neural crest; Neuronal development; Neurons - metabolism; Sequence Homology, Amino Acid; Spinal cord; tag1; Trigeminal ganglion; Zebrafish; Zebrafish - embryology; Zebrafish - genetics; Zebrafish - metabolism; Brain; tag1; Spinal cord; Cell adhesion molecule; Glycoprotein; Neuronal development; Zebrafish; Axon pathfinding; Growth cone; Gene expression; Neural crest; Eye; Lateral line; Trigeminal ganglion; Branchiomotor neuron
• ISSN: 0925-4773; 1872-6356
• DOI: 10.1016/S0925-4773(98)00215-9

TAG-1 is a mammalian cell adhesion molecule of the immunoglobulin superfamily that is expressed transiently by a subset of neurons and serves as a fertile substrate for neurite outgrowth in vitro (Furley, A.H., Morton, S.B., Manalo, D., Karagogeos, S., Dodd, H., Jessell, T.M., 1990 The axonal glycoprotein TAG-1 is an immunoglobulin superfamily member with neurite outgrowth promoting activity. Cell 61, 157–170). In order to examine the in vivo function of this molecule, we have cloned a zebrafish tag1-like cDNA and analyzed its expression patterns. tag1 is expressed transiently by specific subsets of neurons when they are projecting their axons or when they are migrating. The specific and dynamic pattern of expression of zebrafish tag1 is consistent with its proposed role in axon guidance and cell migration.