Bioorthogonal chemistry in bioluminescence imaging

•Bioorthogonal chemistry has broaden the scope of bioluminescence imaging.•Split luciferin based assays allow investigation of aminopeptidase activities.•Azido-modified biomolecules can be imaged using the Staudinger ligation. Bioorthogonal chemistry has developed significant over the past few decad...

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Published inDrug discovery today Vol. 23; no. 9; pp. 1584 - 1590
Main Authors Godinat, Aurélien, Bazhin, Arkadiy A., Goun, Elena A.
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.09.2018
Subjects
Animals
Chemistry Techniques, Synthetic
Firefly Luciferin - analogs & derivatives
Firefly Luciferin - chemical synthesis
Firefly Luciferin - metabolism
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - metabolism
Genes, Reporter
Humans
Luciferases - genetics
Luciferases - metabolism
Luminescent Measurements
Molecular Imaging - methods
Oxidation-Reduction
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Summary:•Bioorthogonal chemistry has broaden the scope of bioluminescence imaging.•Split luciferin based assays allow investigation of aminopeptidase activities.•Azido-modified biomolecules can be imaged using the Staudinger ligation. Bioorthogonal chemistry has developed significant over the past few decades, to the particular benefit of molecular imaging. Bioluminescence imaging (BLI) along with other imaging modalities have significantly benefitted from this chemistry. Here, we review bioorthogonal reactions that have been used to signific antly broaden the application range of BLI.
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content type line 23
ISSN:1359-6446
1878-5832
1878-5832
DOI:10.1016/j.drudis.2018.05.022