Changes in the Microbiota and their Roles in Patients with Type 2 Diabetes Mellitus

• Published in: Current microbiology Vol. 80; no. 4; p. 132
• Main Authors: He, Qiao-ling, Wang, Hui-chao, Ma, Yu-ke, Yang, Rui-lin, Dai, Zhi-feng, Yang, Jing-nan, Xu, Yao, Wei, Dan-dan, Zhu, Li-ping, Lin, Xu-hong
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.04.2023; Springer Nature B.V
• Subjects: Bacteria; bacterial communities; Bacteroides; Bifidobacterium; Biomedical and Life Sciences; Biotechnology; blood glucose; blood serum; Body mass index; Body size; Cholesterol; Community composition; community structure; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2; disease severity; Dysbacteriosis; dysbiosis; Dysbiosis - complications; Enterococcus; Fatty acids; Feces; Glycated Hemoglobin; glycohemoglobin; Hemoglobin; High density lipoprotein; Humans; Insulin Resistance; Intestinal microflora; intestinal microorganisms; Lactic acid; Lactobacilli; Lactobacillus; Life Sciences; Microbiology; Microbiota; noninsulin-dependent diabetes mellitus; Polymerase chain reaction; quantitative polymerase chain reaction; Triglycerides
• ISSN: 0343-8651; 1432-0991; 1432-0991
• DOI: 10.1007/s00284-023-03219-x

An association between type 2 diabetes mellitus (T2DM) and gut microbiota is well established, but the results of related studies are inconsistent. The purpose of this investigation is to elucidate the characteristics of the gut microbiota in T2DM and non-diabetic subjects. Forty-five subjects were recruited for this study, including 29 T2DM patients and 16 non-diabetic subjects. Biochemical parameters, including body mass index (BMI), fasting plasma glucose (FPG), serum total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), and hemoglobin A1c (HbA1c), were analyzed and correlated with the gut microbiota. Bacterial community composition and diversity were detected in fecal samples using direct smear, sequencing, and real-time polymerase chain reaction (PCR). In this study, it was observed that indicators such as BMI, FPG, HbA1c, TC, and TG in T2DM patients were on the rise, concurrent with dysbiosis of the microbiota. We observed an increase in Enterococci and a decrease in Bacteroides , Bifidobacteria, and Lactobacilli in patients with T2DM. Meanwhile, total short-chain fatty acids (SCFAs) and D-lactate concentrations were decreased in the T2DM group. In addition, FPG was positively correlated with Enterococcus and negatively correlated with Bifidobacteria , Bacteroides , and Lactobacilli . This study reveals that microbiota dysbiosis is associated with disease severity in patients with T2DM. The limitation of this study is that only common bacteria were noted in this study, and more in-depth related studies are urgently needed.