Gordonibacter faecis sp. nov., producing urolithin C from ellagic acid, isolated from feces of healthy Korean subjects

• Published in: Archives of microbiology Vol. 206; no. 3; p. 108
• Main Authors: Kim, Han Sol, Kim, Ji-Sun, Suh, Min Kuk, Eom, Mi Kyung, Do, Hyo Eun, Lee, Ju Huck, Park, Seung-Hwan, Kang, Se Won, Lee, Dong Ho, Yoon, Hyuk, Lee, Je Hee, Lee, Jung-Sook
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.03.2024; Springer Nature B.V
• Subjects: Archives & records; bacteria; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; chemotaxonomy; Dimethyl acetals; DNA; Ecology; Ellagic Acid; Fatty acids; Feces; Gene sequencing; Genes; genome; Genomes; genus; Glycolipids; Humans; Hydrolyzable Tannins; Life Sciences; Lipids; Menaquinones; Microbial Ecology; Microbiology; New species; Nucleotide sequence; nucleotide sequences; Original Paper; phenotype; Phospholipids; Phylogenetics; Phylogeny; Polyphenols; Quinones; Republic of Korea; RNA, Ribosomal, 16S - genetics; rRNA 16S; species; Republic of Korea; Gut microbiota; Urolithin C; Human feces; Ellagic acid; Gordonibacter
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-024-03844-5

A Gram-stain-positive, anaerobic, motile, and short rod-shaped bacterium, designated KGMB12511 T , was isolated from the feces of healthy Koreansubjects. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain KGMB12511 T was closely related to Gordonibacter pamelaeae 7-10-1-b T (95.2%). The draft genome of KGMB12511 T comprised 33 contigs and 2,744 protein-coding genes. The DNA G + C content was 59.9% based on whole-genome sequences. The major cellular fatty acids (>10%) of strain KGMB12511 T were C 18:1 cis 9, C 18:1 cis 9 DMA (dimethylacetal), and C 16:0 DMA. The predominant polar lipids included a diphosphatydilglycerol, four glycolipids, and an unidentified phospholipid. The major respiratory quinones were menaquinone 6 (MK-6) and monomethylmenaquinone 6 (MMK-6). Furthermore, HPLC analysis demonstrated the ability of strain KGMB12511 T to convert ellagic acid into urolithin. Based on a comprehensive analysis of phenotypic, chemotaxonomic, and phylogenetic data, strain KGMB12511 T represents a novel species in the genus Gordonibacter . The type strain is KGMB12511 T (= KCTC 25343 T  = NBRC 116190 T ).