Can co-inoculation of Bradyrhizobium and Azospirillum alleviate adverse effects of drought stress on soybean (Glycine max L. Merrill.)?

• Published in: Archives of microbiology Vol. 201; no. 3; pp. 325 - 335
• Main Authors: Silva, Elijanara Raissa, Zoz, Jardel, Oliveira, Carlos Eduardo Silva, Zuffo, Alan Mario, Steiner, Fábio, Zoz, Tiago, Vendruscolo, Eduardo Pradi
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2019; Springer Nature B.V
• Subjects: abortion (plants); adverse effects; Azospirillum brasilense; Azospirillum brasilense - growth & development; Azospirillum brasilense - physiology; Biochemistry; Biomedical and Life Sciences; Biotechnology; Bradyrhizobium - growth & development; Bradyrhizobium - physiology; Bradyrhizobium japonicum; Cell Biology; Cell membranes; Drought; Drought resistance; drought tolerance; Droughts; Ecology; Glycine max; Glycine max - growth & development; Glycine max - microbiology; greenhouses; Inoculation; Irrigation; irrigation management; Leaves; Life Sciences; Microbial Ecology; Microbiology; Moisture content; Nodulation; Original Paper; Plant growth; plant growth-promoting rhizobacteria; Plant Leaves - microbiology; Plant Root Nodulation - physiology; Plant Roots - microbiology; Plants; Side effects; Soybeans; Stability; Stress; Stress, Physiological - physiology; Water - metabolism; Water content; water stress; (L.) Merrill; Plant growth-promoting rhizobacteria; Inoculation; Drought tolerance; Glycine max (L.) Merrill
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-018-01617-5

Harnessing the beneficial potential of plant growth-promoting rhizobacteria may be an alternative strategy to improve plant tolerance to drought stress. The effect of inoculation with Bradyrhizobium japonicum and Azospirillum brasilense either alone or in combination on the plant growth and drought tolerance of soybean [ Glycine max (L.) Merrill.] was investigated in this study in greenhouse conditions. Treatments were arranged in a randomized block design in a 3 × 4 factorial: three irrigation regimes [100% of pot capacity—PC (well-watered control), 50% of PC (moderate stress) and 25% of PC (severe stress)] and four inoculation treatments [control (non-inoculated), inoculation with B. japonicum , inoculation with A. brasilense , and co-inoculation with B. japonicum and A . brasilense ]. Leaf relative water content, cell membrane stability, root nodulation, plant growth, and morphophysiological indexes were recorded. The inoculation of soybean plants with B . japonicum and A. brasilense either alone or in combination improved leaf membrane stability under drought stress conditions when compared to non-inoculated plants; however, this lower damage to cell membranes was not sufficient to maintain the leaf water content of the plant under drought stress. Plants co-inoculated with B . japonicum and A. brasilense improved the root nodulation under severe drought conditions. Inoculation of B . japonicum and A. brasilense either alone or in combination reduced the pod abortion rate under moderate drought stress, but had no effect under severe drought stress. In summary, the co-inoculation of A. brasilense and B. japonicum alleviate adverse effects limited by drought stress to the growth of soybeans.Author: Please check and confirm that the authors [Elijanara Raissa Silva, Carlos Eduardo Silva Oliveira, Alan Mario Zuffo, Eduardo Pradi Vendruscolo] and their initials have been correctly identified and amend if necessary.The authors were correctly identified.