Characterization of Cd-resistant Klebsiella michiganensis MCC3089 and its potential for rice seedling growth promotion under Cd stress

• Published in: Microbiological research Vol. 210; pp. 12 - 25
• Main Authors: Mitra, Soumik, Pramanik, Krishnendu, Ghosh, Pallab Kumar, Soren, Tithi, Sarkar, Anumita, Dey, Ramendra Sundar, Pandey, Sanjeev, Maiti, Tushar Kanti
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.05.2018
• Subjects: ACC deaminase; Bioremediation; Cadmium resistant bacteria; Ethylene; Klebsiella sp; PGPR; MALDI TOF-MS; Cadmium resistant bacteria; Ethylene; Klebsiella sp; FAME; AAS; PGP traits; ACC deaminase; EDX; SEM; Bioremediation; PGPR; TEM
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/j.micres.2018.03.003

Application of heavy metal resistant plant growth promoting rhizobacteria has an important role as they help to evade metal-induced toxicity in plants on one hand and enhance plant growth on the other. The present study is therefore focused on the characterization of a cadmium resistant bacterial strain isolated from heavy metal contaminated rhizospheric soil designated as S8. This S8 strain was selected in terms of cadmium resistance and plant growth promoting traits. Moreover, it also showed resistance to lead and arsenic to a considerable extent. The selected strain S8 was identified as Klebsiella michiganensis by modern approaches of bacterial taxonomy. The plant growth promoting traits exhibited by the strain include 1-aminocyclopropane-1-carboxylic acid deaminase activity (58.33 ng α-keto butyrate/mg protein/h), Indole-3-acetic acid production (671 μg/ml), phosphate solubilization (71.98 ppm), nitrogen fixation (3.72 μg of nitrogen fixed/h/mg protein) etc. Besides, the strain also exhibited high cadmium removal efficiency (73–97%) from the medium and intracellular accumulation as well. Its efficiency to alleviate cadmium-induced toxicity was determined against a rice cultivar in terms of morphological and biochemical changes. Enhanced growth and reduced oxidative stress were detected in presence of the bacterium. On the basis of these results, it can be concluded that K. michiganensis strain S8 is cadmium accumulating plant growth promoting rhizobacterium that can be applied in cadmium contaminated agricultural soil to achieve better productivity of rice.