Modeling and optimization of the process conditions for biomass production and sporulation of a probiotic culture
The present study is aimed at evaluating and analyzing the effects of the critical process parameters by using response surface modeling method and optimizing the same to enhance the yields of biomass and endospores of a probiotic culture. A 24 full factorial central composite design followed by a m...
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Published in | Process biochemistry (1991) Vol. 40; no. 7; pp. 2531 - 2538 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
01.06.2005
|
Subjects | |
Online Access | Get full text |
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Summary: | The present study is aimed at evaluating and analyzing the effects of the critical process parameters by using response surface modeling method and optimizing the same to enhance the yields of biomass and endospores of a probiotic culture. A 24 full factorial central composite design followed by a multistage Monte Carlo optimization was employed for experimental design and analysis of the results and process optimization. The optimal process conditions for maximum biomass production were: pH
=
6.65; temperature
=
38.3
°C; agitation
=
247
rpm and aeration
=
1.05
vvm and those for the maximum sporulation were: pH
=
6.27; temperature
=
41.4
°C; agitation
=
115
rpm and aeration
=
0.33
vvm. Hence, a two-stage strategy with biomass production in exponential phase under the optimal growth conditions in the first stage followed by the second stage in stationary phase under the optimal conditions for sporulation was thus adopted to obtain a maximum probiotic biomass yield of 4.3
g
l
−1 and spore yield of 9
×
10
11 spores
g
−1 of dry biomass for the formulation of effective nutraceuticals. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 1359-5113 1873-3298 |
DOI: | 10.1016/j.procbio.2004.11.004 |