Effects of ketamine administration on the phosphorylation levels of CREB and TrKB and on oxidative damage after infusion of MEK inhibitor

• Published in: Pharmacological reports Vol. 68; no. 1; pp. 177 - 184
• Main Authors: Réus, Gislaine Z., Abaleira, Helena M., Titus, Stephanie E., Arent, Camila O., Michels, Monique, da Luz, Jaine R., dos Santos, Maria Augusta B., Carlessi, Anelise S., Matias, Beatriz I., Bruchchen, Livia, Steckert, Amanda V., Ceretta, Luciane B., Dal-Pizzol, Felipe, Quevedo, João
• Format: Journal Article
• Language: English
• Published: Cham Elsevier Urban & Partner Sp. z o.o 01.02.2016; Springer International Publishing
• Subjects: Aniline Compounds - administration & dosage; Animals; Benzamides - administration & dosage; Brain - drug effects; Brain - metabolism; CREB; Cyclic AMP Response Element-Binding Protein - metabolism; Depression; Drug Safety and Pharmacovigilance; Infusions, Intravenous; Ketamine; Ketamine - pharmacology; Male; MEK; Mitogen-Activated Protein Kinase Kinases - antagonists & inhibitors; Original Research Article; Oxidative stress; Oxidative Stress - drug effects; Oxidative Stress - physiology; Pharmacotherapy; Pharmacy; Phosphorylation - drug effects; Phosphorylation - physiology; Rats; Rats, Wistar; Receptor, trkB - metabolism; Oxidative stress; MEK; CREB; Ketamine; pCREB; pTrKB; NMDA; Depression; TBARS; PFC
• ISSN: 1734-1140; 2299-5684
• DOI: 10.1016/j.pharep.2015.08.010

Ketamine, an antagonist of N-methyl-d-aspartate (NMDA) receptors, has presented antidepressant effects in basic and clinical studies. The MAPK kinase (MEK) signaling pathway could be a target for novel antidepressant drugs and an important pathway involved in neuronal plasticity. Thus, this study evaluated the effects of the administration of ketamine on the phosphorylation of TrKB and CREB, and oxidative stress parameters in the prefrontal cortex (PFC), hippocampus, amygdala, and nucleus accumbens (NAc) rats, after the inhibition of MAPK pathway (PD184161). Male adult Wistar rats were submitted to a surgical procedure to receive a single dose of a pharmacological inhibitor of MAPK (PD184161) at a dose of (0.1μg/μl) or vehicle. Then, they were divided: 1) vehicle+saline; 2) inhibitor PD184161+saline; 3) vehicle+ketamine 15mg/kg; and 4) inhibitor PD184161+ketamine 15mg/kg. MEK inhibitor and ketamine increased the phosphorylation of the transcription factor cAMP response element-binding protein (pCREB) and neurotrophic factor/tropomyosin related kinase B receptor (pTrKB) in the PFC, and decreased pCREB in the hippocampus. The MEK inhibitor abolished ketamine's effects in the hippocampus. In the amygdala, pCREB was decreased, and pTrKB was increased after MEK inhibitor plus ketamine. Ketamine increased the thiobarbituric acid reactive species (TBARS) in the PFC, hippocampus, amygdala, and NAc; MEK inhibitor antagonized these effects. The carbonyl was increased in the PFC by both ketamine and MEK inhibitor, but inhibitor infusion plus ketamine administration reduced this effect. In the amygdala, MEK inhibitor increased carbonyl. Ketamine's effects on pCREB, pTrKB, and oxidative stress are mediated, at least in part, by a mechanism dependent of MAPK signaling inhibition.