N-acetylcysteine Amid Reduces Pancreatic Damage in A Rat Model of Acute Necrotizing Pancreatitis

• Published in: The Journal of surgical research Vol. 203; no. 2; pp. 383 - 389
• Main Authors: Turkyilmaz, Serdar, MD, Usta, Arif, MD, Cekic, Arif Burak, MD, Alhan, Etem, MD, Kural, Birgül Vanizor, PhD, Ercin, Cengiz, MD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.06.2016
• Subjects: Acetylcysteine - analogs & derivatives; Acetylcysteine - pharmacology; Acetylcysteine - therapeutic use; Acute necrotizing pancreatitis; Animals; Antioxidants - pharmacology; Antioxidants - therapeutic use; Biomarkers - metabolism; Ceruletide; Hemodynamics - drug effects; Kidney - drug effects; Kidney - physiopathology; Liver - drug effects; Liver - physiopathology; Male; N-acetylcysteine amid; NACA; Pancreas - drug effects; Pancreas - metabolism; Pancreas - pathology; Pancreas - physiopathology; Pancreatitis, Acute Necrotizing - chemically induced; Pancreatitis, Acute Necrotizing - drug therapy; Pancreatitis, Acute Necrotizing - mortality; Pancreatitis, Acute Necrotizing - physiopathology; Random Allocation; Rats; Rats, Sprague-Dawley; Surgery; Treatment Outcome; N-acetylcysteine amid; NACA; Acute Necrotizing Pancreatitis; Acute necrotizing pancreatitis
• ISSN: 0022-4804; 1095-8673
• DOI: 10.1016/j.jss.2016.03.004

Abstract Background Inflammatory explosion and oxidative stress are important mechanisms of injury in acute necrotizing pancreatitis (ANP). This study investigated the effects of N-acetylcysteine amid (NACA), a novel cell-permeant antioxidant with anti-inflammatory activity, on experimental ANP in rats. Materials and Methods Fifty-two adult male Sprague-Dawley rats were used and ANP was induced by cerulein. The animals were divided into four groups which were sham + saline, sham + NACA, ANP + saline and ANP + NACA. NACA (2.2 mg/kg, i.p) was administered for 6 h, after the induction of ANP. The extent of acinar cell injury, mortality, systemic cardiorespiratory variables, functional capillary density, renal/hepatic functions, and changes in some enzyme markers for pancreas and lung tissues were investigated. Results Induction of ANP increased mortality from 0% in the sham group to 43.75% in the ANP+ saline group ( P <0.05), and administration of NACA significantly reduced mortality to 12.5% (P<0.05). Induction of ANP also caused increases in pancreatic necrosis, serum amylase, ALT, interleukin-6, LDH in bronchoalveolar lavage fluid, serum urea, tissue myeloperoxidase in pancreas and lung tissues and malondialdehyde. There was less pronounced increase in these parameters in NACA treated group. Compared with ANP group, ANP+NACA group had lower levels of pancreatic necrosis (0.5±0.2 vs. 1.45±0.2, P <0.05) and inflammation (0.6±0.2 vs. 1.29±00.3, P < 0.05) scores. Conclusion Administration of NACA significantly decreased the ANP-induced mortality and also provided significant improvements in hemodynamic changes. The obtained positive effects of NACA on the course of pancreatitis indicates its potential usefulness in the management of ANP.