BPA induces hepatotoxicity in zebrafish through oxidative stress and apoptosis pathways

• Published in: Fish physiology and biochemistry Vol. 50; no. 2; pp. 403 - 412
• Main Authors: Wang, Ying, Wu, Jie, Wang, Dagang, Wan, Mengqi, Li, Xue, Zhang, Li, Yang, Dou, Liu, Fasheng, Liu, Jiejun, Li, Kehao, Zhang, Shouhua, Lu, Huiqiang
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.04.2024; Springer Nature B.V
• Subjects: Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; antioxidants; Apoptosis; Aquatic organisms; Astaxanthin; Benzhydryl Compounds - toxicity; Bioengineering; Biological stress; Biomedical and Life Sciences; Bisphenol A; Body length; Bottles; Chemical and Drug Induced Liver Injury; Danio rerio; Freshwater & Marine Ecology; Freshwater fishes; Gene expression; Genes; Hepatocytes; Hepatotoxicity; Histology; Humans; Laboratory animals; Larva; Larvae; Life Sciences; Liver; Malformations; Metabolism; Mineral water; Morphology; mortality; Nutrient uptake; Nutrients; Oxidative Stress; Phenols - toxicity; Toxicity; Toxins; Water Pollutants, Chemical - toxicity; Yolk sac; Zebrafish; Zebrafish - genetics; Zoology; Bisphenol A; Zebrafish larvae; Oxidative stress; Abnormal behavior; Hepatotoxicity; Apoptosis
• ISSN: 0920-1742; 1573-5168; 1573-5168
• DOI: 10.1007/s10695-023-01284-4

BPA is so ubiquitous that 27 million tons of BPA-containing plastic, including mineral water bottles and baby bottles, is produced worldwide each year. The potential toxicity of BPA to humans and aquatic organisms has been the subject of intense research. In this study, a zebrafish model system was used to assess BPA-mediated hepatotoxicity. Zebrafish larvae at 72-144 hpf were exposed to BPA at different concentrations (0,1, 3 and 5mg/L). For example, BPA-treated zebrafish larvae showed increased mortality, delayed uptake of nutrients in yolk sac, shortened body length, smaller liver area, abnormal expression of genes related to liver development, and pathological changes in the liver tissue. Mechanistically, BPA exposure induced excessive oxidative stress in the liver of zebrafish and increased the level of hepatocyte apoptosis in zebrafish larvae, and the antioxidant astaxanthin could rescue the BPA-mediated liver toxicity.