Pathological and molecular identification of porcine cysticercosis in Maharashtra, India

• Published in: Acta parasitologica Vol. 63; no. 4; pp. 784 - 790
• Main Authors: Satyaprakash, Kaushik, Khan, Waqar A., Chaudhari, Sandeep P., Shinde, Shilpshree V., Kurkure, Nitin V., Kolte, Sunil W.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 19.12.2018; De Gruyter; Springer Nature B.V
• Subjects: Animal Systematics/Taxonomy/Biogeography; Animals; Biomedical and Life Sciences; Biomedicine; Brain; Brain - parasitology; Brain - pathology; Calcification; Carcasses; Cysticercosis; Cysticercosis - epidemiology; Cysticercosis - parasitology; Cysticercosis - veterinary; Cysts; Degeneration; Diaphragm; Diaphragm - parasitology; Diaphragm - pathology; DNA, Protozoan - chemistry; DNA, Protozoan - isolation & purification; Ecology; Eggs; Electrophoresis, Agar Gel - veterinary; Eosinophils; Food safety; gene; Gliosis; Health risks; Histopathology; India - epidemiology; Infiltration; Leukocytes (eosinophilic); Liver; Liver - parasitology; Liver - pathology; Medical Microbiology; Meninges; Microbiology; Multiplex Polymerase Chain Reaction - veterinary; Muscle, Skeletal - parasitology; Muscle, Skeletal - pathology; Muscles; Neurodegeneration; neuronal degeneration; Oligonucleotides; Organs; Original Paper; Parasitology; Parenchyma; PCR; perivascular cuffing; pigs; Prevalence; Primers; Public health; RNA, Ribosomal - genetics; rRNA; Skeletal muscle; Suidae; Swine; Swine Diseases - epidemiology; Swine Diseases - parasitology; Swine Diseases - pathology; Taenia solium; Taenia solium - anatomy & histology; Taenia solium - genetics; Taenia solium - isolation & purification; Tongue - parasitology; Tongue - pathology; Zoonoses; Zoonoses - parasitology; India; Maharashtra India; pigs; prevalence; neuronal degeneration; Cysticercosis; gene; perivascular cuffing; PCR; PCR, LSU rRNA gene; Mt-Cox1 gene
• ISSN: 1230-2821; 1896-1851
• DOI: 10.1515/ap-2018-0094

Porcine cysticercosis, caused by metacestodes of Taenia solium is an important emerging zoonotic disease with public health and economic significance. Pigs acquire the disease through consumption of Taenia solium eggs excreted by human tapeworm carriers. The present study was conducted to investigate the prevalence of porcine cysticercosis in Nagpur and Mumbai region of Maharashtra, India by P/M examination of carcasses followed by histopathology of affected organs in infected animals and molecular identification of cysts for confirmation. Out of 1000 pigs examined during slaughter, three pigs were found to be heavily affected with T. solium cysts giving a prevalence of 0.3%. Histological section of brain in infected animals revealed marked vascular congestion of meninges, mild neuronal degeneration, perivascular cuffing and gliosis while the liver showed the infiltration of mononuclear cell, predominantly eosinophils throughout the parenchyma. Some degree of calcification was observed in the cysts lodged in liver while calcification was not evident in case of cysts lodged in brain, tongue, diaphragm and skeletal muscle. Molecular identification by PCR using two sets of oligonucleotide primers against LSU rRNA gene and Mt-Cox1 gene of T. solium confirms the cysts to be that of T. solium . The molecular diagnostics methods have been considered for validation in conjunction with P/M inspections, parasitological and histopathological examinations. The study confirms the presence of porcine cysticercosis in the two regions and demands proper sanitary measures to minimize the risk of infection from zoonoses and food safety point of view.