Differential microRNA expression in cultured palatal fibroblasts from infants with cleft palate and controls

• Published in: European journal of orthodontics Vol. 40; no. 1; pp. 90 - 96
• Main Authors: Schoen, Christian, Glennon, Jeffrey C, Abghari, Shaghayegh, Bloemen, Marjon, Aschrafi, Armaz, Carels, Carine E L, Von den Hoff, Johannes W
• Format: Journal Article
• Language: English
• Published: UK Oxford University Press 23.01.2018
• Subjects: Case-Control Studies; Cells, Cultured; Child, Preschool; Cleft Palate - genetics; Cleft Palate - pathology; Female; Fibroblasts - metabolism; Gene Expression Profiling - methods; Gene Expression Regulation; Humans; Infant; Male; MicroRNAs - genetics; Palate, Hard - metabolism; Palate, Hard - pathology; Real-Time Polymerase Chain Reaction - methods
• ISSN: 0141-5387; 1460-2210; 1460-2210
• DOI: 10.1093/ejo/cjx034

Summary Background The role of microRNAs (miRNAs) in animal models of palatogenesis has been shown, but only limited research has been carried out in humans. To date, no miRNA expression study on tissues or cells from cleft palate patients has been published. We compared miRNA expression in palatal fibroblasts from cleft palate patients and age-matched controls. Material and Methods Cultured palatal fibroblasts from 10 non-syndromic cleft lip and palate patients (nsCLP; mean age: 18 ± 2 months), 5 non-syndromic cleft palate only patients (nsCPO; mean age: 17 ± 2 months), and 10 controls (mean age: 24 ± 5 months) were analysed with next-generation small RNA sequencing. All subjects are from Western European descent. Sequence reads were bioinformatically processed and the differentially expressed miRNAs were technically validated using quantitative reverse-transcription polymerase chain reaction (RT-qPCR). Results Using RNA sequencing, three miRNAs (hsa-miR-93-5p, hsa-miR-18a-5p, and hsa-miR-92a-3p) were up-regulated and six (hsa-miR-29c-5p, hsa-miR-549a, hsa-miR-3182, hsa-miR-181a-5p, hsa-miR-451a, and hsa-miR-92b-5p) were down-regulated in nsCPO fibroblasts. One miRNA (hsa-miR-505-3p) was down-regulated in nsCLP fibroblasts. Of these, hsa-miR-505-3p, hsa-miR-92a, hsa-miR-181a, and hsa-miR-451a were also differentially expressed using RT-PCR with a higher fold change than in RNAseq. Limitations The small sample size may limit the value of the data. In addition, interpretation of the data is complicated by the fact that biopsy samples are taken after birth, while the origin of the cleft lies in the embryonic period. This, together with possible effects of the culture medium, implies that only cell-autonomous genetic and epigenetic differences might be detected. Conclusions For the first time, we have shown that several miRNAs appear to be dysregulated in palatal fibroblasts from patients with nsCLP and nsCPO. Furthermore, large-scale genomic and expression studies are needed to validate these findings.