Covalently Bound Lipids of Human Stratum Corneum

• Published in: Journal of investigative dermatology Vol. 92; no. 1; pp. 109 - 111
• Main Authors: Wertz, Philip W., Madison, Kathi C., Downing, Donald T.
• Format: Journal Article
• Language: English
• Published: Danvers, MA Elsevier Inc 01.01.1989; Nature Publishing
• Subjects: Animals; Biological and medical sciences; Ceramides - analysis; Chromatography, Thin Layer; Epidermis - analysis; Fundamental and applied biological sciences. Psychology; Humans; Lipids - analysis; Psoriasis - metabolism; Swine; Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue; Human; Covalent bond; Lipids; Skin; Stratum corneum
• ISSN: 0022-202X; 1523-1747
• DOI: 10.1111/1523-1747.ep13071317

In the present study, we demonstrate that human stratum corneum contains covalently bound lipids accounting for 1.4% of the dry weight of the tissue. The major component (53.3% of the total by weight) is a ceramide (CER-A) consisting of 30 through 34-carbon ω-hydroxyacids amide-linked to sphingosine. The other hound lipids in human stratum corneum include fatty acids (12.7%), ω-hydroxy acids (9.4%), and a second, more polar, ω-hydroxyacid-containing ceramide (CER-B, 24.8%). The predominant ω-hydroxyacids in both ceramides, as well as the free hydroxyacid fraction, are the 30-carbon saturated and 32- and 34-carbon monoenoic species. The bound fatty acids consist largely of 14 through 22-carbon saturated specks, but significant proportions of monoenoic species and linoleic acid are also present. Psoriatic scale contains a similar total concentration of the same covalently bound lipids, but the proportions of the individual bound lipids are different from those found in normal stratum corneum. It is suggested that the principal function of the covalently bound lipids in human stratum corneum is the formation of a lipid envelope on the outer surface of the keratinized cells.