Effects of Fe-deficient conditions on Fe uptake and utilization in P-efficient soybean

• Published in: Plant physiology and biochemistry Vol. 112; pp. 1 - 8
• Main Authors: Qiu, Wei, Dai, Jing, Wang, Nanqi, Guo, Xiaotong, Zhang, Xiaoli, Zuo, Yuanmei
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.03.2017
• Subjects: Biological Transport; Biomass; Chlorosis; Fe and P interaction; FMN Reductase - metabolism; Gene Expression Regulation, Plant; Glycine max - genetics; Glycine max - growth & development; Glycine max - metabolism; Hydroponic; Iron - metabolism; Iron - pharmacology; Nutrient deficiency; Phosphorus - metabolism; Plant Leaves - drug effects; Plant Leaves - metabolism; Plant Roots - drug effects; Plant Roots - metabolism; Plant Shoots - metabolism; Soil; Soybean; ICP-AES; Nutrient deficiency; SPAD; Soybean; Chlorosis; P; Fe0; Fe-EDTA; DW; Fe80; DAT; Fe and P interaction; Fe40; Fe20; Hydroponic; Fe
• ISSN: 0981-9428; 1873-2690
• DOI: 10.1016/j.plaphy.2016.12.010

Phosphorus (P)-efficient soybean (Glycine max) plants absorb and utilize P with high efficiency. To investigate the effects of iron (Fe)-deficient conditions on the absorption and utilization of Fe in P-efficient soybean plants, two soybean cultivars with different P efficiency, the 03-3 (P-efficient variety) and Bd-2 (P-inefficient variety), were used in this study. The two soybean cultivars were grown in nutrient solution containing Fe concentrations of 0 (Fe0), 20 (Fe20), 40 (Fe40), or 80 (Fe80) μM for 7 days. The Fe reductase activity of roots was higher in 03-3 plants grown under the Fe0, Fe20, and Fe40 treatments than in Bd-2 plants and the total Fe uptake was greater in 03-3 plants under the Fe40 treatment. GmFRD3a was much more highly expressed in the stem of 03-3 than in that of Bd-2, and significantly more iron was transported to 03-3 plant shoots during Fe0 treatment. Chlorosis in young leaves caused by Fe deficiency under the Fe0 and Fe20 treatments was alleviated by increased Fe concentration in shoots. Increased levels of active Fe in young 03-3 leaves under Fe-deprivation conditions (Fe0) and maintenance of stable Fe concentrations in 03-3 shoots subjected to Fe20, Fe40, and Fe80 treatments suggested that the P-efficient 03-3 cultivar is also Fe-efficient. It is suggested that 03-3 soybean cultivar should be a good resource for application to farm field. •We examined the effects of Fe deficiency in two different P-efficient soybeans.•Study the effects in the two soybeans at physiological and molecular level.•03-3 cultivar (P-efficient soybean) could delay the FeP interaction.•Fe distribution was optimized in the 03-3 cultivar under Fe-deficient conditions.•03-3 cultivar soybean would be a valuable variety for agricultural practice.