The usefulness of a single measurement of insulin-like growth factor-1 as a predictor of embryo yield and pregnancy rates in a bovine MOET program

• Published in: Theriogenology Vol. 64; no. 9; pp. 1977 - 1994
• Main Authors: Velazquez, M.A., Newman, M., Christie, M.F., Cripps, P.J., Crowe, M.A., Smith, R.F., Dobson, H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2005
• Subjects: 3-Hydroxybutyric Acid - blood; Animals; Biomarkers - blood; Cattle; Cattle - physiology; Cholesterol - blood; dairy cows; Embryo transfer; Embryo Transfer - veterinary; Embryo, Mammalian; Fatty Acids, Nonesterified - blood; Female; Follicle Stimulating Hormone - administration & dosage; IGF-1; Insemination, Artificial - veterinary; Insulin - blood; Insulin-Like Growth Factor I - analysis; Ovulation; Ovulation Induction - veterinary; Pregnancy; Superovulation; Tissue and Organ Harvesting - methods; Tissue and Organ Harvesting - veterinary; Tissue Donors; Urea - blood; Embryo transfer; IGF-1; Cattle; Superovulation
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2005.05.001

The objective was to determine if a single measurement of plasma insulin-like growth factor-1 (IGF-1) could predict the number of viable embryos obtained from donors and the likelihood of pregnancy in recipients in multiple ovulation and embryo transfer (MOET) programs in cattle. The embryo yields from 101 embryo recoveries were examined in maiden Holstein heifers ( n = 75) and multiparous Holstein cows (lactating cows n = 20, dry cows n = 6). Donors were superstimulated with FSH and embryo recovery was done non-surgically 7 days after artificial insemination. Embryos were classified according to the IETS criteria. Pregnancy rates in 100 maiden Holstein heifer recipients were analysed. Recipients were on day 7 ± 1 of the estrous cycle at transfer. Pregnancy diagnosis was carried out at day 30 (PD30) and rechecked at day 60 (PD60) after transfer. Blood samples from coccygeal vessels taken at the time of embryo recovery (donors) and transfer (recipients) were analysed for IGF-1, insulin, β-hydroxybutyrate (β-OHB), non-esterified fatty acids (NEFA), urea and cholesterol. There was a negative correlation between the number of viable embryos and insulin ( r = −0.33, P = 0.025) in donor heifers. In donor cows, the number of viable embryos was correlated with IGF-1 ( r = 0.43, P = 0.028) and cholesterol ( r = −0.43, P = 0.027). In recipients, PD30 and PD60 were not affected by any of the circulating parameters analysed. Insulin, IGF-1 and cholesterol only explained 8.9, 13.9 and 15.8% of the variation in the production of viable embryos, respectively. Several factors affect MOET programs and under the circumstances of the present study the usefulness of hormonal and metabolic profiles as predictors of the outcome of this biotechnology was limited.