Iraqi propolis increases degradation of IL-1β and NLRC4 by autophagy following Pseudomonas aeruginosa infection

• Published in: Microbes and infection Vol. 20; no. 2; pp. 89 - 100
• Main Authors: Jabir, Majid S., Sulaiman, Ghassan M., Taqi, Zainab J., Li, Dong
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.02.2018
• Subjects: Autophagy; IL-1β; Inflammasome; Iraqi propolis; NLRC4; Pseudomonas aeruginosa; Pseudomonas aeruginosa; Inflammasome; Iraqi propolis; NLRC4; IL-1β; Autophagy
• ISSN: 1286-4579; 1769-714X
• DOI: 10.1016/j.micinf.2017.10.007

Autophagy is a cellular process for maintaining cellular homeostasis. This process can be induced by different factors, such as immune stimuli and pathogen-associated molecules. Autophagy has an important role in the control of IL-1β secretion by macrophages and other cell types. In present study, we describe a novel role for Iraqi propolis affecting autophagy in controlling the secretion of IL-1β in bone-marrow macrophages (BMDMs). After infection with Pseudomonas aeruginosa in the presence of propolis, the degradation of IL-1β was induced, and the activity of inflammasome was reduced. Iraqi propolis-induced autophagy in in vitro and in vivo models decreased the levels of IL-1β and caspase-1. Results indicated that IL-1β pathway production is regulated by autophagy via two different novel mechanisms, namely, regulation of the activation of NLRC4 inflammasome and IL-1β targeting for lysosomal degradation.