Generating mouse models of retinal disease using ENU mutagenesis

We used the chemical mutagen, N-ethyl- N-nitrosourea, to induce random point mutations in the germline of the mouse strain C57BL/6 in order to generate models of retinal diseases. 1163 mutagenised first generation mice produced using this approach were examined for eye abnormalities. Approximately o...

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Bibliographic Details
Published inVision research (Oxford) Vol. 42; no. 4; pp. 479 - 485
Main Authors Baird, P.N, Guymer, R.H, Chiu, D, Vincent, A.L, Alexander, W.S, Foote, S.J, Hilton, D.J
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.02.2002
Subjects
Animals
Breeding
Disease
Ethylnitrosourea
Eye Abnormalities - genetics
Female
Genetics
Germ-Line Mutation
Male
Mice
Mice, Inbred C57BL
Models, Animal
Mouse
Mutagens
Mutant
Phenotype
Point Mutation
Retina
Retinal Diseases
Testis - drug effects
Genetics
Retina
Disease
Mouse
Mutant
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Summary:We used the chemical mutagen, N-ethyl- N-nitrosourea, to induce random point mutations in the germline of the mouse strain C57BL/6 in order to generate models of retinal diseases. 1163 mutagenised first generation mice produced using this approach were examined for eye abnormalities. Approximately one-third (412) presented with some form of ocular abnormality. Most changes were unilateral and confined to the anterior segment of the eye. Less than 10% (44) of identified changes affected the posterior segment of the eye. 21 mice with varying ocular abnormalities, including 17 with retinal changes, were bred to produce second generation mice to confirm genetic inheritance. Genetic inheritance was confirmed in several of these lines including three with retinal changes.
ISSN:0042-6989
1878-5646
DOI:10.1016/S0042-6989(01)00232-2