Complete genome sequence of human T-cell lymphotropic type 1 from patients with different clinical profiles, including infective dermatitis

• Published in: Infection, genetics and evolution Vol. 79; p. 104166
• Main Authors: Araújo, Thessika Hialla Almeida, Barreto, Fernanda Khouri, Menezes, Aline Dórea Luz, Lima, Clayton Pereira Silva de, Oliveira, Rodrigo Santos de, Lemos, Poliana da Silva, Galvão-Castro, Bernardo, Kashima, Simone, Farre, Lourdes, Bittencourt, Achilea Lisboa, Carvalho, Edgar Marcelino de, Santos, Luciane Amorim, Rego, Filipe Ferreira de Almeida, Mota-Miranda, Aline Cristina Andrade, Nunes, Márcio Roberto Teixeira, Alcântara, Luiz Carlos Júnior
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2020
• Subjects: Adolescent; Adult; Aged; Child; Complete genome; Dermatitis - virology; Female; Genetic Variation; Genome Size; Genome, Viral; High-Throughput Nucleotide Sequencing; HTLV-1; Human T-lymphotropic virus 1 - classification; Human T-lymphotropic virus 1 - genetics; Humans; Leukemia-Lymphoma, Adult T-Cell - virology; Male; Middle Aged; Nucleotide variations; Paraparesis, Tropical Spastic - virology; Phylogeny; Whole Genome Sequencing - methods; Young Adult; HTLV-1; Complete genome; Nucleotide variations
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2019.104166

The HTLV-1 is the first human retrovirus and is associated with several clinical syndromes, however, the pathogenesis of these clinical manifestations is still not fully understood. Furthermore, there are few complete genomes publicly available, about 0.12 complete genomes per 10,000 infected individuals and the databases have a major deficiency of sequences information. This study generated and characterized 31 HTLV-1 complete genomes sequences derived from individuals with Tropical Spastic Paraparesis/HTLV-1-Associated Myelopathy (TSP/HAM), Adult T-cell leukemia/lymphoma (ATL), infective dermatitis associated to HTLV-1 (IDH) and asymptomatic patients. These sequences are associated to clinical and epidemiological information about the patients. The sequencing data generated on Ion Torrent PGM platform were assembled and mapped against the reference HTLV-1 genome. These sequences were genotyped as Cosmopolitan subtype, Transcontinental subgroup. We identified the variants in the coding regions of the genome of the different clinical profiles, however, no statistical relation was detected. This study contributed to increase of HTLV-1 complete genomes in the world. Furthermore, to better investigate the contribution of HTLV-1 mutations for the disease outcome it is necessary to evaluate the interaction of the viral genome and characteristics of the human host. •There are few complete genomes publicly available.•The databases have a major deficiency of HTLV-1 sequences of information.•The project generated and characterized 31 complete HTLV-1 genome sequences.