Identification of the adherent microbiota on the gills and skin of poly-cultured gibel carp (Carassius auratus gibelio) and bluntnose black bream (Megalobrama amblycephala Yih)

• Published in: Aquaculture research Vol. 41; no. 9; pp. e72 - e83
• Main Authors: Wang, Wenwen, Zhou, Zhigang, He, Suxu, Liu, Yuchun, Cao, Yanan, Shi, Pengjun, Yao, Bin, Ringø, Einar
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.08.2010; Blackwell Publishing Ltd
• Subjects: Actinobacteria; adherent microbiota; Ascomycota; Carassius auratus gibelio; Firmicutes; Freshwater; gill; Megalobrama amblycephala; PCR-DGGE; poly-culture pond; Proteobacteria; skin
• ISSN: 1355-557X; 1365-2109
• DOI: 10.1111/j.1365-2109.2009.02459.x

PCR-denaturing gradient gel electrophoresis (DGGE) was applied to analyse the microbial community attached to the gills and skin of poly-cultured gibel carp (Carassius auratus gibelio) and bluntnose black bream (Megalobrama amblycephala Yih) and compare these results with those detected in the rearing water. The microbiota discussed included bacteria, fungi and a specific bacterial taxa of actinomycetes was also analysed. Proteobacteria, Firmicutes, Actinobacteria, Cyanobacteria, Ascomycota, Basidiomycota and some unclassified microbiota were identified. Based on our results, we concluded that: (1) the adherent bacterial/fungal communities on the gills and skin were different from those in the rearing water, (2) the bacterial/fungal diversities on fish gills were lower than that on fish skin, (3) the adherent bacterial/fungal communities on gill and skin of gibel carp were different from that of bluntnose black bream and (4) the adherent actinomycetal community showed certain similarity between the skin of different hosts. Based on our conclusions, we suggested that the topic investigated in the present study merits further investigations.