Synthesis, In Silico and In Vitro Evaluation for Acetylcholinesterase and BACE-1 Inhibitory Activity of Some N -Substituted-4-Phenothiazine-Chalcones

• Published in: Molecules (Basel, Switzerland) Vol. 25; no. 17; p. 3916
• Main Authors: Tran, Thai-Son, Le, Minh-Tri, Nguyen, Thi-Cam-Vi, Tran, The-Huan, Tran, Thanh-Dao, Thai, Khac-Minh
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.08.2020; MDPI
• Subjects: acetylcholiesterase inhibitor; Acetylcholinesterase; Alzheimer's disease; BACE-1; Biological activity; chalcone; Chalcones - chemical synthesis; Chalcones - pharmacology; Cholinesterase Inhibitors - chemical synthesis; Cholinesterase Inhibitors - pharmacology; docking; Enzymes; Flavonoids; Hydrogen bonds; in silico; Ligands; Molecular docking; Molecular Docking Simulation; Molecular modelling; NMR; Nuclear magnetic resonance; Phenothiazine; Phenothiazines - chemical synthesis; Phenothiazines - pharmacology; Prediction models; QSAR; Quantitative Structure-Activity Relationship; Secretase; Structure-activity relationships; Substitutes; Two dimensional models; β-Site APP-cleaving enzyme 1; β-Site APP-cleaving enzymes; chalcone; docking; acetylcholiesterase inhibitor; in silico; QSAR; BACE-1
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25173916

Acetylcholinesterase (AChE) and beta-secretase (BACE-1) are two attractive targets in the discovery of novel substances that could control multiple aspects of Alzheimer's disease (AD). Chalcones are the flavonoid derivatives with diverse bioactivities, including AChE and BACE-1 inhibition. In this study, a series of -substituted-4-phenothiazine-chalcones was synthesized and tested for AChE and BACE-1 inhibitory activities. In silico models, including two-dimensional quantitative structure-activity relationship (2D-QSAR) for AChE and BACE-1 inhibitors, and molecular docking investigation, were developed to elucidate the experimental process. The results indicated that 13 chalcone derivatives were synthesized with relatively high yields (39-81%). The bioactivities of these substances were examined with pIC 3.73-5.96 (AChE) and 5.20-6.81 (BACE-1). Eleven of synthesized chalcones had completely new structures. Two substances AC4 and AC12 exhibited the highest biological activities on both AChE and BACE-1. These substances could be employed for further researches. In addition to this, the present study results suggested that, by using a combination of two types of predictive models, 2D-QSAR and molecular docking, it was possible to estimate the biological activities of the prepared compounds with relatively high accuracy.