Potassium and chloride fluxes are involved in volume regulation in mouse pancreatic islet cells

Potassium and chloride transport were measured in beta-cell-rich islets from ob/ob-mice using 36Cl- and 86Rb+ (K+-analogue). Reduction of the osmolarity from the normal 317 mosm l-1 to 180 mosm l-1 reduced the apparent content of K+ and Cl-. Hypo-osmolarity had no effect on the ouabain-sensitive por...

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Bibliographic Details
Published inActa physiologica Scandinavica Vol. 128; no. 4; p. 541
Main Authors Lindström, P, Norlund, L, Sehlin, J
Format Journal Article
LanguageEnglish
Published England 01.12.1986
Subjects
Animals
Biological Transport - drug effects
Chlorides - metabolism
Glyburide - pharmacology
Islets of Langerhans - physiology
Mice
Mice, Obese
Osmolar Concentration
Ouabain - pharmacology
Potassium - metabolism
Tetracaine - pharmacology
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Summary:Potassium and chloride transport were measured in beta-cell-rich islets from ob/ob-mice using 36Cl- and 86Rb+ (K+-analogue). Reduction of the osmolarity from the normal 317 mosm l-1 to 180 mosm l-1 reduced the apparent content of K+ and Cl-. Hypo-osmolarity had no effect on the ouabain-sensitive portion of the Rb+ influx (Na+/K+ pump), but reduced the ouabain-resistant portion of the influx. Hypo-osmolarity also strongly increased the Rb+ efflux rate. Both tetracaine (0.5 mM) and glibenclamide (20 microM), which increase the osmotic resistance of pancreatic beta cells, significantly potentiated the reduction in apparent K+ content induced by hypo-osmolarity. This study suggests that the volume regulation in pancreatic beta cells is partly due to K+ and Cl- flux and that glibenclamide and tetracaine increase the osmotic resistance of the beta cells by affecting such ion transport.
ISSN:0001-6772
DOI:10.1111/j.1748-1716.1986.tb08010.x