Comparison of levels of cytochromes P-450, CYP1A2, CYP2E1, and their related monooxygenase activities in human surgical liver samples

• Published in: Alcoholism, clinical and experimental research Vol. 17; no. 4; p. 900
• Main Authors: Lucas, D, Berthou, F, Dréano, Y, Lozac'h, P, Volant, A, Ménez, J F
• Format: Journal Article
• Language: English
• Published: England 01.08.1993
• Subjects: Adult; Aged; Aged, 80 and over; Cytochrome P-450 CYP1A2; Cytochrome P-450 Enzyme System - metabolism; Female; Humans; Liver - enzymology; Liver - pathology; Liver Diseases, Alcoholic - enzymology; Liver Diseases, Alcoholic - pathology; Male; Middle Aged; Oxidoreductases - metabolism; Oxygenases - metabolism; Smoking - adverse effects; Smoking - pathology
• ISSN: 0145-6008
• DOI: 10.1111/j.1530-0277.1993.tb00861.x

Hepatic microsomal cytochromes P-450 CYP1A2, and CYP2E1 contents and catalytic activities have been simultaneously investigated in 42 patients undergoing diagnostic liver biopsy. CYP1A2 contents, measured by Western blotting, were correlated with methoxyresorufin-O-demethylation and ethoxyresorufin-O-deethylation (r = 0.65 and r = 0.66, p < 0.001, respectively). CYP2E1 contents were correlated with 1-butanol oxidation and 6-hydroxylation of chlorzoxazone (r = 0.75 for both, p < 0.001). CYP1A2 catalytic activities varied by 30- to 40-fold, whereas CYP2E1 activities varied by 6- to 20-fold. In our study, these variations were not related to liver diseases or cancer of the digestive tract nor to alcohol drinking or smoking habits, because patients were alcohol- and tobacco-free for 1 month before the study. Other environmental factors, diet habits, and/or genetic factors could explain the large interindividual variations observed.