Alkaline Extraction, Structural Characterization, and Bioactivities of (1→6)-β-d-Glucan from Lentinus edodes

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 8; p. 1610
• Main Authors: Li, Jia, Cai, Chao, Zheng, Mengmeng, Hao, Jiejie, Wang, Ya, Hu, Minghua, Fan, Luodi, Yu, Guangli
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.04.2019; MDPI
• Subjects: (1→6)-β- d -glucan; Anion exchanging; antioxidant; Antioxidants; Aqueous solutions; Atomic force microscopy; Biocompatibility; Biological properties; Carbon; Caustic soda; Cell activation; Cytotoxicity; Design of experiments; Design optimization; Experimental design; Functional foods & nutraceuticals; Glucan; Glucans; immunomodulatory activities; Lentinus edodes; Light scattering; Liquid chromatography; Macrophages; Magnetic resonance spectroscopy; Methylation; Molecular weight; Monosaccharides; Nitric oxide; NMR; NMR spectroscopy; Nuclear magnetic resonance; orthogonal optimization; Polysaccharides; Precipitation; Refractivity; Saccharides; solution conformation; Spectrum analysis; Structural analysis; structure characterization; β-Glucan; antioxidant; solution conformation; orthogonal optimization; structure characterization; (1→6)-β-d-glucan; Lentinus edodes; immunomodulatory activities
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24081610

The purpose of this study is to develop a robust approach to obtain β glucans from and to characterize their structural and biological properties for sustainable utilization. The alkali extraction was optimized with an orthogonal experimental design, and a concise process for obtaining specific targeting polysaccharides from was developed in this study. After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations. LeP-N2 was found to be composed of (1→6)-β-d-glucans with minor β-(1→3) glucosidic side chains. Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution. This (1→6)-β-d-glucan was tested for antioxidant activities with IC at 157 μg/mL. Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) was markedly increased with no cytotoxicity at a dose of 100 μg/mL. These findings suggest that the (1→6)-β-d-glucans obtained from could serve as potential agents in the fields of functional foods or medicine.