MiR-129-5p influences the progression of gastric cancer cells through interacting with SPOCK1

• Published in: Tumor biology Vol. 39; no. 6; p. 1010428317706916
• Main Authors: Yan, Lei, Sun, Kai, Liu, Yang, Liang, Jun, Cai, Kerui, Gui, Jinqiu
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.06.2017; Sage Publications Ltd
• Subjects: Apoptosis; Apoptosis - genetics; Authorship; Cell cycle; Cell Cycle Checkpoints - genetics; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Cell proliferation; Cell Proliferation - genetics; Cell Survival - genetics; Dehydrogenases; Disease Progression; Drug resistance; Female; Flow cytometry; Gastric cancer; Gene Expression Regulation, Neoplastic; Growth factors; Humans; Invasiveness; Investigations; Kinases; Male; Metastasis; MicroRNAs - genetics; miRNA; Mitosis; Neoplasm Invasiveness - genetics; Neoplasm Invasiveness - pathology; Pathogenesis; Polymerase chain reaction; Proteoglycans - biosynthesis; Proteoglycans - genetics; Reporter gene; Stomach Neoplasms - genetics; Stomach Neoplasms - pathology; Tumor cell lines; United States > US; SPOCK1; mitosis; Gastric cancer; microRNA-129-5p; viability
• ISSN: 1010-4283; 1423-0380
• DOI: 10.1177/1010428317706916

The purpose of our study is to clarify the effect of microRNA-129-5p in the progression of human gastric cancer cells by regulating SPOCK1. The expression of microRNA-129-5p and SPOCK1 was tested by quantitative real-time polymerase chain reaction in tissues and cell lines. We validated the targeted relationship between microRNA-129-5p and SPOCK1 by dual luciferase reporter gene assay. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation, flow cytometry, transwell, and wound scratch assays were used to analyze the effects of microRNA-129-5p on SGC-7901 cell viability, proliferation, cell cycle and apoptosis, invasiveness, and migration. MicroRNA-129-5p was downregulated while SPOCK1 was upregulated in gastric cancer tissues and cell lines. The result of luciferase reporter gene assay demonstrated that microRNA-129-5p can target SPOCK1 by binding to the 3′untranslated region. The overexpression of microRNA-129-5p or the inhibition of SPOCK1 inhibited SGC-7901 viability, proliferation, migration, and invasion while promoted cell cycle arrest in G0/G1 stage and cell apoptosis. Our results suggested that microRNA-129-5p could directly specifically suppress SPOCK1, which might be one of the potential mechanisms in inhibiting cell processes including viability, proliferation, cell mitosis, migration, and invasiveness of gastric cancer cells.