Solid-state and polymer nanopores for protein sensing: A review

• Published in: Advances in colloid and interface science Vol. 298; p. 102561
• Main Authors: Meyer, Nathan, Abrao-Nemeir, Imad, Janot, Jean-Marc, Torrent, Joan, Lepoitevin, Mathilde, Balme, Sebastien
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2021; Elsevier
• Subjects: Chemical Sciences; Ionic diode; Nanochannel; Nanopore; Protein sensing; Resistive pulse; Single molecule; Nanochannel; Nanopore; Protein sensing; Resistive pulse; Ionic diode; Single molecule
• ISSN: 0001-8686; 1873-3727
• DOI: 10.1016/j.cis.2021.102561

In two decades, the solid state and polymer nanopores became attractive method for the protein sensing with high specificity and sensitivity. They also allow the characterization of conformational changes, unfolding, assembly and aggregation as well the following of enzymatic reaction. This review aims to provide an overview of the protein sensing regarding the technique of detection: the resistive pulse and ionic diodes. For each strategy, we report the most significant achievement regarding the detection of peptides and protein as well as the conformational change, protein-protein assembly and aggregation process. We discuss the limitations and the recent strategies to improve the nanopore resolution and accuracy. A focus is done about concomitant problematic such as protein adsorption and nanopore lifetime. [Display omitted] •The protein and peptide sensing by solid state and polymer nanopore was reviewed regarding the method of detection.•We discuss the recent achievement to analyse the conformational change, protein-protein assembly and aggregation process•We discuss the limitation and strategy to improve the protein detection