Expression of Lung Surfactant Proteins SP-B and SP-C and Their Modulating Factors in Fetal Lung of FGR Rats

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 35; no. 1; pp. 122 - 128
• Main Author: 邓飞涛 欧阳为相 葛良芳 张莉 柴新群
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.02.2015; Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
• Subjects: Animals; Base Sequence; DNA Primers; Female; Fetal Growth Retardation; Lung - embryology; Lung - metabolism; Medicine; Medicine & Public Health; Peptides - metabolism; Pregnancy; Pulmonary Surfactant-Associated Protein B - metabolism; Rats; Real-Time Polymerase Chain Reaction; SP-C; Western印迹; 大鼠模型; 活性蛋白; 肺发育; 胎儿; 表面; 调节因子; Western blot; SP-B; fetal growth restriction; SP-C; PLAGL2; real-time PCR; immunohistochemistry; TTF-1; lung development
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-015-1400-y

This study investigated the expression of lung surfactant proteins SP-B and SP-C, and their modulating factors TTF-1 and PLAGL2 in the fetal lung of rats with fetal growth restriction（FGR）. The rat FGR model was established by prenatal hypoxia in the first stage of pregnancy, 180 rats for experiment served as hypoxia group, and 197 healthy rats served as normal control group. The FGR incidence in hypoxia was compared with that in normal control group. The histological changes in the fetal lung were observed under the light microscope and electronic microscope in two groups. The SP-B, SP-C, TTF-1 and PLAGL2 proteins were determined in the fetal lung of two groups immunohistochemically. The expression levels of SP-B, SP-C, TTF-1 and PLAGL2 protein and m RNA in the fetal lung of two groups were detected by using Western blotting and RT-PCR respectively. The FGR rat model was successfully established by using hypoxia. Pathologically the fetal lung developed slowly, and the expression levels of SP-B, SP-C, TTF-1 and PLAGL2 protein and mR NA in the fetal lung were significantly reduced in hypoxia group as compared with those in normal control group. It was suggested that maternal hypoxia in the first stage of pregnancy could induce FGR, and reduce the expression of SP-B and SP-C, resulting in the disorder of fetal lung development and maturation.