Digital In-Line Holography as a Three-Dimensional Tool to Study Motile Marine Organisms During Their Exploration of Surfaces

• Published in: The Journal of adhesion Vol. 83; no. 5; pp. 417 - 430
• Main Authors: Heydt, M., Rosenhahn, A., Grunze, M., Pettitt, M., Callow, M. E., Callow, J. A.
• Format: Journal Article
• Language: English
• Published: Basingstoke Taylor & Francis Group 23.05.2007; Taylor and Francis
• Subjects: Biodeterioration. Biofouling; Biofouling; Biological and medical sciences; Biotechnology; Digital in-line holography; Exploration pattern; Fundamental and applied biological sciences. Psychology; Gabor; Industrial applications and implications. Economical aspects; Surface exploration; Three-dimensional tracking; Ulva linza; 3D imaging; Digital in-line holography; Experimental study; Exploration pattern; Surface exploration; Three-dimensional tracking; Seaweeds; Particle mobility; Spores; Investigation method; Biofouling; Optical holography; Surface properties; Ulva linza; Formation mechanism; Gabor
• ISSN: 0021-8464; 1563-518X; 1545-5823
• DOI: 10.1080/00218460701377388

The swimming patterns of zoospores of the green alga Ulva linza in the vicinity of a surface were investigated by digital in-line holography. Full 3D motion patterns were retrieved from measurements and the traces obtained were compared with known swimming patterns of spores of the brown alga Hincksia irregularis and the green alga Ulva linza as seen in a conventional optical microscope. Quantitative information such as swimming velocity was calculated from the 3D traces. The results demonstrate the potential of digital in-line holography to image and quantify exploratory patterns of behavior of motile spores close to surfaces. This technique can give detailed insight into mechanisms of surface colonization by spores and larvae of fouling organisms in response to changes in surface properties.