Over-expression of long non-coding RNA ZEB2-AS1 may predict poor prognosis and promote the migration, invasion, and epithelial-mesenchymal transition of tumor cells in non-small cell lung cancer

• Published in: The International journal of biological markers Vol. 35; no. 3; pp. 29 - 35
• Main Authors: Xu, Hui, Yu, Bengtong, Shen, Weiyu, Jin, Chenghua, Wang, Lijie, Xi, Yong
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.09.2020; Sage Publications Ltd
• Subjects: Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - metabolism; Carcinoma, Non-Small-Cell Lung - pathology; Cell migration; Cell Movement - physiology; Cell Proliferation - physiology; Cell survival; Cell viability; Epithelial-Mesenchymal Transition; Humans; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Medical prognosis; Mesenchyme; Metastasis; Neoplasm Invasiveness; Neoplasm Metastasis; Non-coding RNA; Non-small cell lung carcinoma; Overexpression; Polymerase chain reaction; Prognosis; RNA, Antisense - genetics; RNA, Antisense - metabolism; RNA, Long Noncoding - biosynthesis; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; siRNA; Small cell lung carcinoma; Survival analysis; Transfection; Tumor cells; Zinc Finger E-box-Binding Homeobox 1 - genetics; ZEB2-AS1; NSCLC; metastasis; prognosis; EMT
• ISSN: 1724-6008; 0393-6155; 1724-6008
• DOI: 10.1177/1724600820938385

Background: Non-small cell lung cancer (NSCLC) remains the most common cause of human cancer-related death worldwide, and the present study aims to explore the roles of long non-coding (lnc)RNA ZEB2-AS1 in NSCLC and the related mechanism. Methods: Quantitative real-time-polymerase chain reaction was performed to compare the expressions of ZEB2-AS1 in NSCLC cancer tissue and the adjacent non-tumorous tissues. The diagnostic and prognostic roles of ZEB2-AS1 in NSCLC were also evaluated by the receiver operating characteristic curve and the Kaplan–Meier survival analysis. NSCLC cell line A549 cells were transfected with ZEB2-AS1 siRNA, and the cell viability, migration, invasion, and epithelial-mesenchymal transition (EMT) of the ZEB2-AS1 siRNA group and control group were compared. Results: ZEB2-AS1 was significantly increased in NSCLC tissues. The knockdown of ZEB2-AS1 markedly inhibited the cell viability, migration, invasion, and EMT of A549 cells in vitro. Conclusion: ZEB2-AS1 was up-regulated in NSCLC, and it may serve as a potential target for the diagnosis, prognosis, and treatment of NSCLC.