Drought Resistance Physiological Responses of Alfalfa to Alternate Partial Root-Zone Drying Irrigation

• Published in: Agriculture (Basel) Vol. 15; no. 13; p. 1446
• Main Authors: Sun, Qunce, Wang, Ying, Zhang, Shuzhen, Peng, Xianwei, Ge, Xingyu, Wen, Binghan, An, Youping, Jin, Guili, Zhang, Yingjun
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.07.2025
• Subjects: Abscisic acid; Agricultural production; Alfalfa; Analysis; Antioxidants; Arid regions; Arid zones; China; Communication; Comparative analysis; Crops; Drought; drought adaptation; Drought resistance; Droughts; Drying; Efficiency; Enzymatic activity; Enzyme activity; Experiments; Growth conditions; Irrigation; Irrigation water; Leaves; Medicago sativa L; Moisture content; physiological response; Physiological responses; Physiology; Rhizosphere; Soil stresses; Water; Water availability; Water conservation; Water deficit; water deficit management; Water resources; Water shortages; Water supply; Water use; Water use efficiency; China
• ISSN: 2077-0472; 2077-0472
• DOI: 10.3390/agriculture15131446

In arid agricultural production, exploring suitable water-saving irrigation strategies and analyzing their water-saving mechanisms are of great significance. Alternating partial root-zone drying irrigation (APRI), a water-saving strategy, enhances the water use efficiency (WUE) of alfalfa (Medicago sativa L.) This paper aims to clarify the physiological mechanisms by which the APRI method enhances the physiological WUE of alfalfa, as well as the differences between this water-saving irrigation strategy, conventional irrigation (CI), and their water deficit adjustments, in order to seek higher water use efficiency for alfalfa production in arid regions. In this experiment, alfalfa was used as the research subject, and three irrigation methods, CI, fixed partial root-zone drying (FPRI), and APRI, were set up, each paired with three decreasing moisture supply gradients of 90% water holding capacity (WHC) (W1), 70% WHC (W2), and 50% WHC (W3). Samples were taken and observed once after every three complete irrigation cycles. Through a comparative analysis of the growth status, leaf water status, antioxidant enzyme activity, and osmotic adjustment capabilities of alfalfa under different water supplies for the three irrigation strategies, the following conclusions were drawn: First, the APRI method, through artificially created periodic wet–dry cycles in the rhizosphere soil, provides pseudo-drought stress that enhances the osmotic adjustment capabilities and antioxidant enzyme activity of alfalfa leaves during the early to middle phases of irrigation treatment compared to CI and FPRI methods, resulting in healthier leaf water conditions. Secondly, the stronger drought tolerance and superior growth conditions of alfalfa under the APRI method due to reduced water availability are key factors in enhancing the water use efficiency of alfalfa under this strategy.