Proton motive force dissipation precludes interaction of microcin J25 with RNA polymerase, but enhances reactive oxygen species overproduction

• Published in: Biochimica et biophysica acta Vol. 1790; no. 10; pp. 1307 - 1313
• Main Authors: Dupuy, Fernando G., Chirou, María V. Niklison, de Arcuri, Beatriz Fernández, Minahk, Carlos J., Morero, Roberto D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2009
• Subjects: 2,4 DNP; 2,4-Dinitrophenol - pharmacology; Amino Acid Substitution; Anti-Bacterial Agents - metabolism; Anti-Bacterial Agents - pharmacology; Bacteriocins - genetics; Bacteriocins - metabolism; Bacteriocins - pharmacology; Cell Membrane - drug effects; Cell Membrane - metabolism; DNA-Directed RNA Polymerases - metabolism; E. coli; Escherichia coli - drug effects; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Escherichia coli Proteins - pharmacology; Gene Expression Regulation, Bacterial - drug effects; Microbial Sensitivity Tests; Microcin; Mutation; Oxygen Consumption - drug effects; Peptide uptake; Protein Binding - drug effects; Proton-Motive Force; Reactive oxygen species; Reactive Oxygen Species - metabolism; Uncoupling Agents - pharmacology; 2,4 DNP; Reactive oxygen species; Peptide uptake; Microcin; E. coli

Microcin J25 targets the RNA polymerase as well as bacterial membranes. Because there is scarce information on the relationship between the uptake and the activity, a fluorescent microcin J25-derivative was used to further characterize its mechanism of action. MccJ25 I13K was labeled with FITC and i...