Possible participation of macrophage inflammatory protein 2 in neutrophil infiltration in allergic inflammation in rats

• Published in: Biochimica et biophysica acta Vol. 1361; no. 2; pp. 138 - 146
• Main Authors: Xiao, Yi-Qun, Tanabe, Jun-ichi, Edamatsu, Takeo, Hirasawa, Noriyasu, Mue, Suetsugu, Ohuchi, Kazuo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 22.08.1997
• Subjects: Amino Acid Sequence; Animals; Anti-macrophage inflammatory protein 2 antibody; Antibodies - administration & dosage; Antibodies - immunology; Antigen-Antibody Reactions; Body Fluids - immunology; Cheek; Chemokine; Chemokine CXCL2; Chemotactic activity; Chemotactic Factors - administration & dosage; Chemotactic Factors - immunology; Cloning, Molecular; Hypersensitivity, Immediate - immunology; Hypersensitivity, Immediate - physiopathology; Inflammation - immunology; Inflammation - physiopathology; Macrophage inflammatory protein 2; Molecular Sequence Data; Monokines - administration & dosage; Monokines - immunology; Neutrophil infiltration; Neutrophils - physiology; Rat allergic inflammation; Rats; Recombinant Proteins - immunology; Chemokine; Neutrophil infiltration; Chemotactic activity; Macrophage inflammatory protein 2; Rat allergic inflammation; Anti-macrophage inflammatory protein 2 antibody
• ISSN: 0925-4439; 0006-3002; 1879-260X
• DOI: 10.1016/S0925-4439(97)00034-3

Recombinant rat macrophage inflammatory protein 2 (MIP-2) was prepared from E. coli transfected with a glutathione- S-transferase (GST)-MIP-2 fusion protein expression vector. A polyclonal antibody to rat MIP-2 was then obtained from rabbits by immunization with recombinant rat MIP-2. Using the polyclonal antibody which selectively suppressed neutrophil chemotactic activity of MIP-2, the role of MIP-2 in neutrophil infiltration in allergic inflammation in rats was studied. In an air pouch-type allergic inflammation model in rats, neutrophil infiltration into the pouch fluid increased with time after antigen challenge. Neutrophil chemotactic activity in the pouch fluid collected 8 h after antigen challenge was diminished by anti-MIP-2 antibody. In addition, when leukocytes that had infiltrated into the pouch fluid collected 4 h after antigen challenge were incubated, neutrophil chemotactic activity in the conditioned medium increased time-dependently, and the activity was neutralized by anti-MIP-2 antibody. Furthermore, when anti-MIP-2 antibody was injected into the pouch 6 h after antigen challenge, neutrophil infiltration into the pouch fluid during the next 2 h was suppressed. These findings indicate that MIP-2 plays an important role in neutrophil infiltration in rat allergic inflammation.