Biliverdin Protects against Cisplatin-induced Apoptosis of Renal Tubular Epithelial Cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 36; no. 1; pp. 48 - 52
• Main Author: 吕倩 姚颖 王伟 熊薇 廖文慧
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.02.2016; Department of Hospital Infection Control,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China%Department of Nephrology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China%Department of Hepatic Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China%Department of Geriatrics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
• Subjects: Animals; Antioxidants - pharmacology; Apoptosis; Biliverdine - pharmacology; Cell Line; Cisplatin - toxicity; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Kidney Tubules - cytology; Medicine; Medicine & Public Health; Rats; Reactive Oxygen Species - metabolism; 临床; 医学; 症状; 诊断; apoptosis; cisplatin; biliverdin; reactive oxygen species
• ISSN: 1672-0733; 1993-1352; 1993-1352
• DOI: 10.1007/s11596-016-1540-8

Biliverdin（BV） has long been thought to be a cytotoxic metabolic waste product. It has also been demonstrated to have important cytoprotective functions during oxidative stress. The present study aimed to examine the cytoprotective effect of BV on NRK-52 E cells, a proximal tubular cell line derived from rat kidney. Cells were treated with 50 μmol/L cisplatin for 24 h（cisplatin group） or pre-treated with BV for 30 min, then with 50 μmol/L cisplatin for 24 h（cisplatin＋BV group）. Those given no treatment served as a control. Cell apoptosis was evaluated by flow cytometry and cell viability by Cell Counting Kit-8（CCK-8）. The protein expressions of cleaved caspase3, Bax and Bcl-2 were assessed by Western blotting. Reactive oxygen species（ROS） levels were measured using carboxydichlorodihydrofluorescein diacetate（H2DCF）. The results showed that cisplatin induced the apoptosis of NRK-52 E cells, decreased cell viability, and increased the formation of ROS by upregulating the expression of cleaved caspase3 and Bax and decreasing Bcl-2 protein expression. These effects could be significantly reversed by pretreatment with BV. It was concluded that BV can protect against cisplatin-induced cell apoptosis through the anti-oxidative effects.