The Effects of Root Extract Ruellia tuberosa L on Histopathology and Malondialdehyde Levels on the Liver of Diabetic Rats

• Published in: IOP conference series. Materials Science and Engineering Vol. 299; no. 1; pp. 12022 - 12027
• Main Authors: Laily Kurniawati, Alfin Nur, Aulanni'am, Srihardyastutie, Arie, Safitri, Anna
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.01.2018
• Subjects: Blood; Body weight; Diabetes; Dosage; Free radicals; Glucose; Hexanes; Histology; Histopathology; Malondialdehyde; Rats; Rodents
• ISSN: 1757-8981; 1757-899X
• DOI: 10.1088/1757-899X/299/1/012022

The aim of this research is to study antidiabetic activity of root extract of Ruellia tuberosa L on rats (Rattus novergicus) induced by multiple-low dose streptozotocin as animal diabetic models. The parameters investigated were blood glucose levels, free radicals (MDA, malondialdehyde) levels and hepatic histopathology. The main materials used were n-hexane root extracts from Ruellia tuberosa L. Three groups of rats, including control group (group I), diabetic group (group II), and therapy group with Ruellia tuberosa L (group III), were used. Streptozotocin was given at multiple-low dose of 20 mg/kg of body weight for 5 times in 5 consecutive days i.p. to rats in groups II and III. The Ruellia tuberosa L extracts were then given orally for group III in the dose of 250 mg/kg of body weight per day for 3 weeks. Results of the current work showed that root extract Ruellia tuberosa L had lowered blood glucose levels on rats in group III by 60.3%, from 299.7 ± 24.7 mg/dL up to 119.0 ± 26.6 mg/dL. Moreover, the antidiabetic activity of Ruellia tuberosa L extracts also deduced from decrease of MDA levels in group III, from 3.5 ± 0.3 μg/mL up to 1.7 ± 0.4 μg/mL. The recovery of hepatic organ from treatment group has also been proven from the its histology profiles stained with hematoxylin-eosin.