Culture Media Influenced Laboratory Outcomes But Not Neonatal Birth Weight in Assisted Reproductive Technology

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 35; no. 6; pp. 932 - 937
• Main Authors: Yin, Tai-lang, Zhang, Yi, Li, Sai-jiao, Zhao, Meng, Ding, Jin-li, Xu, Wang-ming, Yang, Jing
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.12.2015; Center of Reproductive Medicine, Renmin Hospital of Wuhan University, Wuhan 430060, China
• Subjects: Birth Weight; Culture Media; Female; Fertilization in Vitro; Humans; Infant, Newborn; Male; Medicine; Medicine & Public Health; Pregnancy; Pregnancy Outcome; 出生体重; 培养基; 多元线性回归分析; 媒体; 实验室; 技术类型; 新生儿; 辅助生殖; birth weight; embryo development; fertilization; culture medium
• ISSN: 1672-0733; 1993-1352; 1993-1352
• DOI: 10.1007/s11596-015-1531-1

Summary： Whether the type of culture media utilized in assisted reproductive technology has impacts on laboratory outcomes and birth weight of newborns in in-vitro fertilization （IVF）/intracytoplasmic sperm injection （ICSI） was investigated. A total of 673 patients undergoing IVF/ICSI and giving birth to live singletons after fresh embryo transfer on day 3 from Jan. 1, 2010 to Dec. 31, 2012 were included. Three types of culture media were used during this period： Quinn＇s Advantage （QA）, Single Step Medium （SSM）, and Continuous Single Culture medium （CSC）. Fertilization rate （FR）, normal fertilization rate （NFR）, cleavage rate （CR）, normal cleavage rate （NCR）, good-quality embryo rate （GQER） and neonatal birth weight were compared using one-way ANOVA and Z2 tests. Multiple linear regression analysis was performed to determine the impact of culture media on laboratory outcomes and birth weight. In IVF cycles, GQER was significantly decreased in SSM medium group as compared with QA or CSC media groups （63.6% vs. 69.0% in QA; vs. 71.3% in CSC, P=0.011）. In ICSI cycles, FR, NFR and CR were significantly lower in CSC medium group than in other two media groups. No significant difference was observed in neonatal birthweight among the three groups （P=0.759）. Multiple linear regression analyses confirmed that the type of culture medium was correlated with FR, NFR, CR and GQER, but not with neonatal birth weight. The type of culture media had potential influences on laboratory outcomes but did not exhibit an impact on the birth weight of singletons in ART.