Genome mining of actinomycin shunt products from Kitasatospora sp. YINM00002

• Published in: RSC advances Vol. 13; no. 51; pp. 36200 - 36208
• Main Authors: Zhang, Zhou-Tian-Le, Sun, Hui-Bing, Ren, Zhen, Xie, Tian-Peng, Wang, Ying-Fang, Guo, Yin, Su, Xiaoyu, Yin, Min, Zhou, Hao, Ding, Zhong-Tao
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 08.12.2023; The Royal Society of Chemistry
• Subjects: Actinomycetes; Actinomycin; Anticancer properties; Biosynthesis; Chemistry; Natural products
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/D3RA07277K

Actinomycins are known for their anti-tumor, antibacterial and antiviral activities, and in particular for the ability of actinomycin D as a clinical drug to treat a variety of cancers. In our ongoing work to obtain novel natural products from endophytic actinomycetes derived from traditional Chinese herbs, we identified the potential to produce actinomycins in YINM00002, a Kitasatospora strain derived from Polygonatum kingianum . According to genome mining, we isolated actinomycins D and V (1 and 2) and small amounts of 4-methyl-3-hydroxyanthranilic acid (4-MHA) derivates (3 and 4) from strain fermentation broth. The presence of actinrhater A (3) and actinrhater B (4) reveals a mysterious shunt pathway in the early stages of actinomycin D biosynthesis. Our study provides a fresh perspective for further discovery and modification of novel actinomycins.