The K2-type killer toxin- and immunity-encoding region from Saccharomyces cerevisiae: structure and expression in yeast

• Published in: Gene Vol. 111; no. 1; pp. 135 - 139
• Main Authors: Meskauskas, A, Citavicius, D
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.02.1992; Amsterdam Elsevier; New York, NY
• Subjects: Aflatoxins - genetics; Aflatoxins - metabolism; Amino Acid Sequence; amino acid sequences; Base Sequence; Biological and medical sciences; cloning; Cloning, Molecular; Codon; codon usage; DNA, Fungal; embl/x54154; Fundamental and applied biological sciences. Psychology; gene structure; genes; Genes. Genome; genetic code; K2 toxin; killer factor; Killer Factors, Yeast; killer phenotype; killer phenotypes; M2 double-stranded RNA; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; mycotoxins; Mycotoxins - genetics; Mycotoxins - metabolism; nucleotide sequence; nucleotide sequences; Phenotype; plant proteins; Recombinant DNA; resistance; RNA; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - immunology; Saccharomyces cerevisiae Proteins; gene structure; aa; killer phenotype; ORF; nt; M2 double-stranded RNA; M2-1; Recombinant DNA; bp; ds; nucleotide sequence; S; kb; cDNA; cloning; Yeast; Double stranded RNA; Nucleotide sequence; Homology; Gene expression; Immunity; Fungi; Toxin; Complementary DNA; Ascomycetes; Molecular cloning; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(92)90615-V

The cDNA copies of M2-1, the larger heat-cleavae product of M2 double-stranded (ds) RNA, have been synthesized, cloned, sequenced and expressed in yeast. This sequence, in combination with the known terminal sequence of M2-1 dsRNA, identifies a translation reading frame for a 362-amino-acid protein of 38.7 kDa, similar in size to the one of several protein species produced from M2-1 dsRNA in vitro translation. The expression of this cDNA clone in yeast confers both killer and immunity phenotypes.