Transgene integration in plants: poking or patching holes in promiscuous genomes?

Transgene integration in plants transformed by either Agrobacterium or direct DNA delivery methods occurs through illegitimate recombination (IR). The precise mechanism(s) for IR-mediated transgene integration and the role of host double-strand break repair enzymes remain unknown. A recent wealth of...

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Bibliographic Details
Published inCurrent opinion in biotechnology Vol. 15; no. 2; pp. 126 - 131
Main Authors Somers, David A, Makarevitch, Irina
Format Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.04.2004
Subjects
Agrobacterium
DNA - genetics
DNA - metabolism
DNA Repair
DNA, Bacterial
Gene Targeting - methods
Gene Transfer Techniques
Genome, Plant
Plants, Genetically Modified
Transgenes
ss
SSA
DSB
DD-DNA
IR
SDSA
HR
NHEJ
ds
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Summary:Transgene integration in plants transformed by either Agrobacterium or direct DNA delivery methods occurs through illegitimate recombination (IR). The precise mechanism(s) for IR-mediated transgene integration and the role of host double-strand break repair enzymes remain unknown. A recent wealth of sequenced transgene loci and investigations aimed at genetically dissecting transgene integration mechanism(s) have provided new insights into the process.
Bibliography:ObjectType-Article-2
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ISSN:0958-1669
1879-0429
DOI:10.1016/j.copbio.2004.02.007