Molecular Identification of Taenia Tapeworms by Cox1 Gene in Koh Kong, Cambodia

• Published in: Korean journal of parasitology Vol. 49; no. 2; pp. 195 - 197
• Main Authors: Jeon, H.K., Chungbuk National University School of Medicine, Cheongju, Republic of Korea, Yong, T.S., Yonsei University College of Medicine, Seoul, Republic of Korea, Sohn, W.M., Gyeongsang National University College of Medicine, Jinju, Republic of Korea, Chai, J.Y., Seoul National University College of Medicine, Seoul, Republic of Korea, Hong, S.J., Chung-Ang University College of Medicine, Seoul, Republic of Korea, Han, E.T., Gangwon University College of Medicine, Chuncheon, Republic of Korea, Jeong, H.G., Korea Association of Health Promotion, Seoul, Republic of Korea, Chhakda, Tep, National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia, Sinuon, Muth, National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia, Socheat, Duong, National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia, Eom, K.S., Chungbuk National University School of Medicine, Cheongju, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한기생충학열대의학회 01.06.2011; The Korean Society for Parasitology
• Subjects: Adolescent; Adult; Animals; Brief Communication; Cambodia; Child; Cyclooxygenase 1 - genetics; DNA Primers - genetics; DNA, Helminth - chemistry; DNA, Helminth - genetics; egg DNA; Feces - parasitology; Female; Helminth Proteins - genetics; Humans; Male; Middle Aged; molecular survey; multiplex PCR; Polymerase Chain Reaction; Sequence Analysis, DNA; TAENIA SAGINATA; Taenia saginata - enzymology; Taenia saginata - genetics; Taenia saginata - isolation & purification; TAENIA SOLIUM; Taenia solium - enzymology; Taenia solium - genetics; Taenia solium - isolation & purification; Taeniasis - parasitology; Young Adult; Cambodia; multiplex PCR; Taenia saginata; Taenia solium; molecular survey; egg DNA
• ISSN: 0023-4001; 1738-0006
• DOI: 10.3347/kjp.2011.49.2.195

We collected fecal samples from 21 individuals infected with Taenia tapeworms in Koh Kong Province, Cambodia, and performed nucleotide sequencing of the cox1 gene and multiplex PCR on the eggs for DNA differential diagnosis of human Taenia tapeworms. Genomic DNA was extracted from the eggs of a minimum number of 10 isolated from fecal samples. Using oligonucleotide primers Ta7126F, Ts7313F, Tso7466F, and Rev7915, the multiplex PCR assay proved useful for differentially diagnosing Taenia solium, Taenia saginata, and Taenia asiatica based on 706, 629, and 474 bp bands, respectively. All of the Taenia specimens from Kho Kong, Cambodia, were identified as either T. saginata (n=19) or T. solium (n=2) by cox1 sequencing and multiplex PCR.