The Prolonged Treatment of Salmonella enterica Strains with Human Serum Effects in Phenotype Related to Virulence

• Published in: International journal of molecular sciences Vol. 24; no. 1; p. 883
• Main Authors: Futoma-Kołoch, Bożena, Małaszczuk, Michał, Korzekwa, Kamila, Steczkiewicz, Małgorzata, Gamian, Andrzej, Bugla-Płoskońska, Gabriela
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 03.01.2023; MDPI AG
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; antibiotics; C1q; C3c; Disinfectants - pharmacology; human serum; Humans; Phenotype; resistance; Salmonella enterica; Salmonella typhimurium - genetics; Virulence; Salmonella enterica; C1q; C3c; hydrophobicity; membrane proteins; biofilm; quaternary ammonium salt; motility; antibiotics; human serum; resistance
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24010883

Salmonella enterica as common pathogens of humans and animals are good model organisms to conduct research on bacterial biology. Because these bacteria can multiply in both the external environments and in the living hosts, they prove their wide adaptability. It has been previously demonstrated that prolonged exposition of Salmonella serotype O48 cells to normal human serum led to an increase in resistance to sera in connection with the synthesis of very long O-antigen. In this work, we have studied the phenotype connected to virulence of Salmonella enterica strains that were subjected to consecutive passages in 50% human serum from platelet-poor plasma (SPPP). We found that eight passages in SPPP may not be enough for the bacteria to become serum-resistant (S. Typhimurium ATCC 14028, S. Senftenberg). Moreover, C1q and C3c complement components bound to Salmonellae (S. Typhimurium ATCC 14028, S. Hammonia) membrane proteins, which composition has been changed after passaging in sera. Interestingly, passages in SPPP generated genetic changes within gene fljB, which translated to cells’ motility (S. Typhimurium ATCC 14028, S. Erlangen). One strain, S. Hammonia exposed to a serum developed a multi-drug resistance (MDR) phenotype and two S. Isaszeg and S. Erlangen tolerance to disinfectants containing quaternary ammonium salts (QAS). Furthermore, colonial morphotypes of the serum adaptants were similar to those produced by starter cultures. These observations suggest that overcoming stressful conditions is manifested on many levels. Despite great phenotypic diversity occurring after prolonged exposition to SPPP, morphotypes of colonies remained unchanged in basic media. This work is an example in which stable morphotypes distinguished by altered virulence can be confusing during laboratory work with life-threatening strains.