The Effect of Aptamer Concetration towards Reduced Graphene Oxide-Field Effect Transistor Surface Channel for Biosensor Application

Aptamer are artificially produce bioreceptor that has been developed to bind with various target biomolecules such as ion, cells, protein and small molecules. In this research, an aptamer concentration of 0.5 nM, 1 nM, 5 nM, 10 nM, and 50 nM were immobilized on reduced graphene oxide (rGO) integrate...

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Published inIOP conference series. Materials Science and Engineering Vol. 318; no. 1; pp. 12045 - 12052
Main Authors Zainol Abidin, Azrul Syafiq, Rahim, Ruslinda Abdul, Huan, Chow Yong, Mohd Maidin, Nur Nasyifa, Ahmad, Nurul Atiqah, Ba Hashwan, Saeed S., Mohd Faudzi, Fatin Nabilah, Hong, Voon Chun
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 19.03.2018
Subjects
Biomolecules
Biosensors
Carboxyl group
Field effect transistors
Graphene
Semiconductor devices
Transistors
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Summary:Aptamer are artificially produce bioreceptor that has been developed to bind with various target biomolecules such as ion, cells, protein and small molecules. In this research, an aptamer concentration of 0.5 nM, 1 nM, 5 nM, 10 nM, and 50 nM were immobilized on reduced graphene oxide (rGO) integrated with field effect transistor (FET) respectively to study the effect of aptamer concentration toward rGO surface for stable biosensing platform. The 0.5 nM concentration of aptamer shows the highest current result of 84.3 µA at 1 V applied through the source and drain. After immobilized with aminated aptamer, the conductivity shows significant reduction due to the formation of amide bond on rGO surface between aminated aptamer and carboxyl group on rGO. The electrical performance of FET integrated with rGO shows stable electrical performance suitable to be used in the biosensing application.
ISSN:1757-8981
1757-899X
DOI:10.1088/1757-899X/318/1/012045