Arabidopsis assay for mutagenicity

• Published in: Mutation research Vol. 310; no. 2; p. 249
• Main Authors: Gichner, T. (Institute of Experimental Botany, Prague (CZECH REPUBLIC)), Badayev, S.A, Demchenko, S.I, Relichova, J, Sandhu, S.S, Usmanov, P.D, Usmanova, O, Veleminsky, J
• Format: Journal Article
• Language: English
• Published: Netherlands 16.10.1994
• Subjects: Arabidopsis - embryology; Arabidopsis - genetics; ARABIDOPSIS THALIANA; AZIDA SODICA; AZIDE DE SODIUM; Azides - toxicity; BIOASSAYS; Biological Assay - methods; CHEMICALS; DOSAGE BIOLOGIQUE; EMS; ENSAYO BIOLOGICO; Environmental Monitoring - methods; Ethyl Methanesulfonate - toxicity; HIDRACIDA MALEICA; HYDRAZIDE MALEIQUE; International Cooperation; LABORATOIRE; LABORATORIES; LABORATORIOS; Maleic Hydrazide - toxicity; Methylnitrosourea - toxicity; MUTAGENE; Mutagenicity Tests - methods; MUTAGENOS; MUTAGENS; Mutagens - analysis; Mutagens - toxicity; PRODUCTOS QUIMICOS; PRODUIT CHIMIQUE; Propylene Glycols - toxicity; Reproducibility of Results; SAFETY; SECURITE; SEGURIDAD; SODIUM AZIDE
• ISSN: 0027-5107; 1873-135X
• DOI: 10.1016/0027-5107(94)90117-1

Four laboratories, two in the Czech Republic (Brno and Prague) and two in the CIS (Moscow and Duschanbe), participated in the International Programme on Chemical Safety's (IPCS) collaborative study to evaluate the utility of the most commonly used plant test systems, including the Arabidopsis thaliana assay, for assessing the mutagenic potential of environmental agents. Out of the five compounds evaluated in the Arabidopsis assay, three compounds, i.e., ethyl methanesulfonate, N-methyl-N-nitrosourea, and azidoglycerol, were reported to be mutagenic by all four participating laboratories. Sodium azide (NaN3) demonstrated a negative response in all four laboratories, whereas maleic hydrazide was reported to be weakly mutagenic by one laboratory and nonmutagenic by the other three laboratories.