Improved Transformation Method for Acetobacter with Plasmid DNA

An improved method for transformation of derivative strains of A. aceti subsp. aceti No. 1023 with plasmid DNA has been developed. Addition of polyethylene glycol or dimethylsulfoxide increased the transformation efficiency by a factor of about ten. In the presence of PEG 4,000, various transformati...

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Published inAgricultural and biological chemistry Vol. 49; no. 7; pp. 2091 - 2097
Main Authors Fukaya, Masahiro, Tayama, Kenji, Okumura, Hajime, Masai, Hiroshi, Uozumi, Takeshi, Beppu, Teruhiko
Format Journal Article
LanguageEnglish
Published Tokyo Taylor & Francis 01.01.1985
Agricultural Chemical Society of Japan
Subjects
Acetobacter aceti
Biological and medical sciences
cloning
Fermented food industries
Food industries
Fundamental and applied biological sciences. Psychology
transformation
Wines and vinegars
Bacteria
Genetics
Mutation
Acetobacter
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Summary:An improved method for transformation of derivative strains of A. aceti subsp. aceti No. 1023 with plasmid DNA has been developed. Addition of polyethylene glycol or dimethylsulfoxide increased the transformation efficiency by a factor of about ten. In the presence of PEG 4,000, various transformation conditions were examined. Cells were also made transformation competent by treatment with other divalent cations than Ca 2+ . The pH of the buffer did not affect the efficiency significantly. The growth phase influenced the efficiency. Mutants showing high competence were derived by treatment with N-methyl-N′-nitro-N-nitrosoguanidine. By the improved method using the highly transformable mutants, a transformation efficiency of approximately 105 transformants per γg plasmid DNA was achieved.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0002-1369
DOI:10.1080/00021369.1985.10867039