Plasma concentrations but not serum concentrations of brain-derived neurotrophic factor are related to pro-inflammatory cytokines in patients undergoing major abdominal surgery

• Published in: Clinical biochemistry Vol. 45; no. 9; pp. 631 - 636
• Main Authors: Chimienti, G., Mezzapesa, A., Rotelli, M.T., Lupo, L., Pepe, G.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.06.2012; Elsevier
• Subjects: Abdomen; Acute-phase response; Adult; Aged; Aged, 80 and over; BDNF; Biological and medical sciences; Blood Platelets - immunology; Brain-Derived Neurotrophic Factor - blood; Cytokines; Cytokines - blood; Cytokines - immunology; Digestive System Surgical Procedures; Female; Humans; Inflammation - blood; Inflammation - immunology; Interleukin-6 - blood; Interleukin-6 - genetics; Interleukin-6 - immunology; Male; Medical sciences; Middle Aged; Perioperative Period; Plasma; Platelet Activation; Polymorphism, Single Nucleotide; Serum; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the digestive system; Surgical stress; Transforming Growth Factor beta1 - blood; Transforming Growth Factor beta1 - immunology; Tumor Necrosis Factor-alpha - blood; Tumor Necrosis Factor-alpha - immunology; Surgical stress; Acute-phase response; Cytokines; BDNF; Human; Cytokine; Biological marker; Inflammation; Blood plasma; Interleukin 6; Clinical biology; Serum; Brain derived neurotrophic factor; Transforming growth factor β1; Comparative study; Tumor necrosis factor α; Quantitative analysis
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2012.02.025

To investigate peripheral brain-derived neurotrophic factor (BDNF) concentrations in the perioperative period, their relationship with transforming growth factor-β1 (TGF-β1 tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-6 genetics. Prospective, observational study. BDNF, TGF-β1, IL-6 and TNF-α were analysed at baseline (T0), 5h (T1), 24h (T2) and 5days (T3) after surgery, in 21 patients. The IL-6 −174 G/C polymorphism was genotyped. Serum BDNF concentrations decreased (P=0.048), correlated with TGF-β1 (r=0.610 at T1, r=0.493 at T2, r=0.554 at T3). Plasma BDNF concentrations raised (P=0.049), correlated with IL-6 and TNF-α at T1 (r=0.495 and r=0.441, respectively). BDNF response was predictable from TNF-α and IL-6 concentrations and the IL-6 −174 G/C genotype. Serum and plasma BDNF concentrations could relate to platelet activation and inflammatory response, respectively. IL-6 genetics played a role in the BDNF acute response.