Decreased anti-Müllerian hormone concentration in follicular fluid of female smokers undergoing artificial reproductive techniques

• Published in: Chemosphere (Oxford) Vol. 88; no. 4; pp. 403 - 406
• Main Authors: Fuentes, Ariel, Muñoz, Alex, Pommer, Ricardo, Argüello, Begoña, Galleguillos, Andrea, Torres, Andrea, Jesam, Cristian
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.07.2012; Elsevier
• Subjects: Adult; AMH; Animal, plant and microbial ecology; Anti-Mullerian Hormone - metabolism; Applied ecology; Biological and medical sciences; Ecotoxicology, biological effects of pollution; Female; Follicular fluid; Follicular Fluid - metabolism; Fundamental and applied biological sciences. Psychology; General aspects; Humans; In vitro fertilization; Reproductive Techniques, Assisted; Smoking; Smoking - metabolism; Time Factors; FF; CS; In vitro fertilization; BS; CN; Follicular fluid; AMH; CP; ES; Smoking; Concentration measurement; Reproduction; Fertilization; Female; Sex steroid hormone; In vitro; Organic compounds
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2012.02.054

► We compared the AMH levels between current smokers and non-smokers in an IVF cycle. ► Current smokers have higher levels of cotinine and lower levels of AMH in the follicular fluid than non-smokers. ► This association had no statistic interaction with age and BMI. Several reports indicate that women who smoke have an increased risk of failure to conceive compared with their non-smoker counterparts. Here, we assessed the effect of smoking during the Assisted Reproduction Therapy (ART) on a potential marker of ovarian reserve, anti-müllerian hormone (AMH) in the follicular fluid (FF). This was a cohort prospective study to assess the association between cigarette smoking and AMH concentrations in FF in fifty-six women undergoing their first ART cycle. Self-reported smoking status over time was also collected through personal interview. The main outcome measured was the association between current smoking and AMH concentrations in FF. Smoking status was assessed by FF cotinine concentrations. Analysis of covariance was performed to test statistical interaction between the main outcome and confounders. The mean concentration of AMH in follicular fluid was significantly decreased among smokers (1.02±0.14 vs. 1.74±0.15, P<0.05). No statistical interaction was found between this difference in AMH concentrations and confounders like age and BMI. Thus, our data support the idea that AMH is decreased in active smokers across the fertile age. The hypothesis of decreased AMH concentration in follicular fluid in female smokers was confirmed. The mechanisms through which cigarette smoking induces this fall in AMH are unknown and additional research is needed to improve our comprehension of the negative impact of smoking on ART outcomes.