Detection of Microvesicle miRNA Expression in ALL Subtypes and Analysis of Their Functional Roles

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 34; no. 5; pp. 640 - 645
• Main Author: 李雯英 陈小梅 熊薇 郭冬梅 卢力 李慧玉
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.10.2014; Neonatal Screening Center, Zhuzhou Women and Children Medical Care Center, Zhuzhou 412000, China%Center for Stem Cell Research and Application, Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Center for Stem Cell Research and Application, Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
• Subjects: Gene Expression Profiling; Gene Expression Regulation, Leukemic; Humans; Jurkat Cells; Jurkat细胞; Medicine; Medicine & Public Health; MicroRNAs - genetics; miRNA; Multivesicular Bodies - genetics; Oligonucleotide Array Sequence Analysis; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - genetics; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - pathology; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma - genetics; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma - pathology; Reverse Transcriptase Polymerase Chain Reaction; signal; 亚型; 微泡; 检测; 白血病细胞; 职能作用; miRNA expression profiles; microvesicles; acute lymphoblastic leukemia subtypes
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-014-1330-0

Microvesicles （MVs） are the heterogeneous mixtures of vesicles. MVs released by leukemia cells constitute an important part of the leukemia microenvironment. MVs might act as important reser- voirs of microRNAs （miRNAs）. It is worth evaluating whether MVs possess some unique miRNA con- tents that are valuable in understanding the pathogenesis. In this study, we investigated the miRNA ex- pression patterns of Nalm-6-derived MVs, Jurkat-derived MVs and normal cell-derived MVs using miRNA microarrays. The potential target genes regulated by differentially expressed miRNAs were also predicted and analyzed. Results demonstrated that 182 miRNAs and 166 miRNAs were differentially expressed in Nalm-6-MVs and Jurkat-MVs, respectively. Many oncogenes, tumor suppressors and sig- nal pathway genes were targeted by these aberrantly expressed miRNAs, which might contribute to the development of B-ALL or T-ALL. Our findings expanded the potential diagnostic markers of ALL and provided useful information for ALL pathogenesis.