Long Non-coding RNA MEG3 Induces Renal Cell Carcinoma Cells Apoptosis by Activating the Mitochondrial Pathway

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 35; no. 4; pp. 541 - 545
• Main Authors: Wang, Miao, Huang, Tao, Luo, Gang, Huang, Chao, Xiao, Xing-yuan, Wang, Liang, Jiang, Guo-song, Zeng, Fu-qing
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.08.2015; Department of Urology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,China%Department of Urology, The First Hospital of Wuhan, Wuhan 430022, China
• Subjects: Apoptosis; Bcl-2; Carcinoma, Renal Cell - genetics; Carcinoma, Renal Cell - metabolism; Carcinoma, Renal Cell - pathology; Cell Line, Tumor; Cell Survival; Gene Expression Regulation, Neoplastic; HEK293 Cells; Humans; Kidney Neoplasms - genetics; Kidney Neoplasms - metabolism; Kidney Neoplasms - pathology; Medicine; Medicine & Public Health; Mitochondria - genetics; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Signal Transduction; 人胚肾细胞; 凋亡途径; 激活; 癌细胞; 线粒体; 诱导; 非编码RNA; MEG3; apoptosis; long non-coding RNA (LncRNA); renal cell carcinoma
• ISSN: 1672-0733; 1993-1352; 1993-1352
• DOI: 10.1007/s11596-015-1467-5

Summary： This study aimed to examine the effect of long non-coding RNA （LncRNA） MEG3 on the biological behaviors of renal cell carcinoma （RCC） cells 786-0 and the possible mechanism. MEG3 expression levels were detected by RT-qPCR in Rmaor tissues and adjacent non-tumor tissues from 29 RCC patients and in RCC lines 786-0 and SN12 and human embryonic kidney cell line 293T. Plasmids GV144-MEG3 （MEG3 overexpression plasmid） and GV144 （control plasmid） were stably transfected into 786-0 cells by using lipofectamine 2000. Cell viabilities were determined by MTT, cell apoptosis rates by flow cytometry following PE Annexin V and 7AAD staining, apoptosis-related protein expressions by Western blotting, and Bcl-2 mRNA by RT-qPCR in the transfected cells. The results showed that MEG3 was evidently downregulated in RCC tissues （P〈0.05） and RCC cell lines （P〈0.05）. The viabilities of 786-0 cells were decreased significantly after transfection with GV144-MEG3 for over 24 h （P〈0.05）. Consistently, the apoptosis rate was significantly increased in 786-0 cells transfected with GV144-MEG3 for 48 h （P〈0.05）. Furthermore, overexpression of MEG3 could reduce the expression of Bcl-2 and procaspase-9 proteins, enhance the expression of cleaved caspase-9 protein, and promote the release of cytochrome c protein to cytoplasm （P〈0.05）. Additionally, Bcl-2 mRNA level was declined by MEG3 overexpression （P〈0.05）. It was concluded that MEG3 induces the apoptosis of RCC cells possibly by activating the mitochondrial pathway.